1kzf
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= EsaI/EsaR gene cluster ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66271 Pantoea stewartii subsp. stewartii]) | |GENE= EsaI/EsaR gene cluster ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66271 Pantoea stewartii subsp. stewartii]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1k4j|1K4J]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kzf OCA], [http://www.ebi.ac.uk/pdbsum/1kzf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kzf RCSB]</span> | ||
}} | }} | ||
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[[Category: quorum sensing]] | [[Category: quorum sensing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:55:47 2008'' |
Revision as of 18:55, 30 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | EsaI/EsaR gene cluster (Pantoea stewartii subsp. stewartii) | ||||||
| Related: | 1K4J
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI
Overview
Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed.
About this Structure
1KZF is a Single protein structure of sequence from Pantoea stewartii subsp. stewartii. Full crystallographic information is available from OCA.
Reference
Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing., Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME, Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774
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