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4wx0
From Proteopedia
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| - | ''' | + | ==UndA complexed with beta-hydroxydodecanoic acid== |
| + | <StructureSection load='4wx0' size='340' side='right' caption='[[4wx0]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wx0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WX0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HXD:(3R)-3-HYDROXYDODECANOIC+ACID'>HXD</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wwj|4wwj]], [[4wwz|4wwz]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wx0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wx0 RCSB], [http://www.ebi.ac.uk/pdbsum/4wx0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aliphatic medium-chain 1-alkenes (MCAEs, approximately 10 carbons) are "drop-in" compatible next-generation fuels and precursors to commodity chemicals. Mass production of MCAEs from renewable resources holds promise for mitigating dependence on fossil hydrocarbons. An MCAE, such as 1-undecene, is naturally produced by Pseudomonas as a semivolatile metabolite through an unknown biosynthetic pathway. We describe here the discovery of a single gene conserved in Pseudomonas responsible for 1-undecene biosynthesis. The encoded enzyme is able to convert medium-chain fatty acids (C10-C14) into their corresponding terminal olefins using an oxygen-activating, nonheme iron-dependent mechanism. Both biochemical and X-ray crystal structural analyses suggest an unusual mechanism of beta-hydrogen abstraction during fatty acid substrate activation. Our discovery unveils previously unidentified chemistry in the nonheme Fe(II) enzyme family, provides an opportunity to explore the biology of 1-undecene in Pseudomonas, and paves the way for tailored bioconversion of renewable raw materials to MCAE-based biofuels and chemical commodities. | ||
| - | + | Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase.,Rui Z, Li X, Zhu X, Liu J, Domigan B, Barr I, Cate JH, Zhang W Proc Natl Acad Sci U S A. 2014 Dec 8. pii: 201419701. PMID:25489112<ref>PMID:25489112</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cate, J D.H]] | ||
| + | [[Category: Li, X]] | ||
| + | [[Category: 1-undecene biosynthesis]] | ||
| + | [[Category: Aliphatic medium-chain 1-alkene biosynthesis]] | ||
| + | [[Category: Metalloenzyme]] | ||
| + | [[Category: Non-heme iron dependent desaturase/decarboxylase]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 10:52, 24 December 2014
UndA complexed with beta-hydroxydodecanoic acid
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