1lka
From Proteopedia
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|PDB= 1lka |SIZE=350|CAPTION= <scene name='initialview01'>1lka</scene>, resolution 1.70Å | |PDB= 1lka |SIZE=350|CAPTION= <scene name='initialview01'>1lka</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1lkb|1LKB]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lka OCA], [http://www.ebi.ac.uk/pdbsum/1lka PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lka RCSB]</span> | ||
}} | }} | ||
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[[Category: Tucker, P A.]] | [[Category: Tucker, P A.]] | ||
[[Category: Weiss, M S.]] | [[Category: Weiss, M S.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: CL]] | ||
[[Category: acetate binding]] | [[Category: acetate binding]] | ||
[[Category: beta-barrel]] | [[Category: beta-barrel]] | ||
| Line 36: | Line 36: | ||
[[Category: chymotrypsin-fold]] | [[Category: chymotrypsin-fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:03:54 2008'' |
Revision as of 19:03, 30 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Pancreatic elastase, with EC number 3.4.21.36 | ||||||
| Related: | 1LKB
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Porcine Pancreatic Elastase/Ca-Complex
Overview
Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.
About this Structure
1LKA is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Metal binding to porcine pancreatic elastase: calcium or not calcium., Weiss MS, Panjikar S, Nowak E, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1407-12. Epub 2002, Aug 23. PMID:12198296
Page seeded by OCA on Sun Mar 30 22:03:54 2008
