Cellulosomal scaffolding protein
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
== Carbohydrate Binding Module (Family 3 Clostridium Thermicellum)== | == Carbohydrate Binding Module (Family 3 Clostridium Thermicellum)== | ||
| - | <StructureSection load='1NBC' size='340' side='right' caption=' | + | <StructureSection load='1NBC' size='340' side='right' caption='Cellulosomal scaffolding protein A complex with Ca+2 ions (PDB code [[1nbc]])' scene=''> |
Carbohydrate-binding domain modules(CBM) are non-catalytic domain which present in glycoside hydrolases which target the associated catalytic modules to their substraes, therefore potentiating enzyme activietvarious microrganisms. CBM3 is part of CipA scafolding in Cellulosome which contain enzyme with hydrolytic activity(cellulase). <ref>doi:10.1016/j.pep.2008.01.018</ref> | Carbohydrate-binding domain modules(CBM) are non-catalytic domain which present in glycoside hydrolases which target the associated catalytic modules to their substraes, therefore potentiating enzyme activietvarious microrganisms. CBM3 is part of CipA scafolding in Cellulosome which contain enzyme with hydrolytic activity(cellulase). <ref>doi:10.1016/j.pep.2008.01.018</ref> | ||
Revision as of 09:09, 29 February 2016
Carbohydrate Binding Module (Family 3 Clostridium Thermicellum)
| |||||||||||
References
- ↑ Guerreiro CI, Fontes CM, Gama M, Domingues L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expr Purif. 2008 May;59(1):161-8. doi: 10.1016/j.pep.2008.01.018. Epub, 2008 Feb 5. PMID:18328729 doi:http://dx.doi.org/10.1016/j.pep.2008.01.018
- ↑ Boraston AB, Bolam DN, Gilbert HJ, Davies GJ. Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J. 2004 Sep 15;382(Pt 3):769-81. PMID:15214846 doi:http://dx.doi.org/10.1042/BJ20040892
- ↑ Guillen D, Sanchez S, Rodriguez-Sanoja R. Carbohydrate-binding domains: multiplicity of biological roles. Appl Microbiol Biotechnol. 2010 Feb;85(5):1241-9. doi: 10.1007/s00253-009-2331-y., Epub 2009 Nov 12. PMID:19908036 doi:http://dx.doi.org/10.1007/s00253-009-2331-y
- ↑ Wan W, Wang D, Gao X, Hong J. Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast. Appl Microbiol Biotechnol. 2011 Aug;91(3):789-98. doi: 10.1007/s00253-011-3373-5., Epub 2011 Jun 9. PMID:21656139 doi:http://dx.doi.org/10.1007/s00253-011-3373-5
