Endonuclease
From Proteopedia
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*HpyI ENN | *HpyI ENN | ||
| - | **[[3fc3]], [[3gox – HpENN99 + DNA – ''Helicobacter pylori''<br /> | + | **[[3fc3]], [[3gox]] – HpENN99 + DNA – ''Helicobacter pylori''<br /> |
**[[3or3]] – HpENN88 + DNA | **[[3or3]] – HpENN88 + DNA | ||
Revision as of 13:23, 24 November 2014
Template:STRUCTURE 1rva
Endonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN. See also
3D structures of endonuclease
Updated on 24-November-2014
