This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Gcn4
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
{{STRUCTURE_2dgc| PDB=2dgc | SIZE=400| SCENE= |right|CAPTION=Gcn4 complex with DNA [[2dgc]] }} | {{STRUCTURE_2dgc| PDB=2dgc | SIZE=400| SCENE= |right|CAPTION=Gcn4 complex with DNA [[2dgc]] }} | ||
| - | '''Gcn4''' is a yeast transcriptional activator. It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a DNA recognition motif | + | '''Gcn4''' is a yeast transcriptional activator. It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a DNA recognition motif consisting of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induces the transition of this region from unstructured to α-helical. Gcn4-pLI is a 33-residue peptide of the dimerization domain of Gcn4. See also [[Intrinsically Disordered Protein]]. |
== 3D Structures of Gcn4 == | == 3D Structures of Gcn4 == | ||
| Line 8: | Line 8: | ||
{{#tree:id=OrganizedByTopic|openlevels=0| | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
| - | *Gcn4 | + | *Gcn4 coiled-coil domain |
| - | **[[2zta]], [[1w5i]], [[2b1f]], [[2b22]], [[2ag3]], | + | **[[2zta]], [[1w5i]], [[2b1f]], [[2b22]], [[2ag3]], [[2r2v]], [[4c46]], [[4hu5]], [[4hu6]] – yGcn4 - yeast <br /> |
**[[2ovn]], [[2l56]] – yGcn4 - NMR<br /> | **[[2ovn]], [[2l56]] – yGcn4 - NMR<br /> | ||
| - | **[[1u2u]] – yGcn4 dimer - NMR<br /> | ||
| - | **[[4dmd]], [[1kd8]], [[1kd9]], [[1kdd]], [[4hu5]], [[4hu6]] – yGcn4 dimer <br /> | ||
| - | **[[4dme]] – yGcn4 trimer <br /> | ||
**[[3ck4]], [[3crp]], [[2ipz]] – yGcn4 tetramer <br /> | **[[3ck4]], [[3crp]], [[2ipz]] – yGcn4 tetramer <br /> | ||
| - | **[[1gcl]], [[1swi]], [[1ce9]], [[1w5g]], [[1w5h]], [[1w5j]], [[1w5k]], [[1w5l]], [[1unt]], [[1unu]], [[1unv]], [[1unw]], [[1unx]], [[1uny]], [[1unz]], [[1uo0]], [[1uo1]], [[1uo2]], [[1uo3]], [[1uo4]], [[1uo5]], [[1u9f]], [[1u9g]], [[1u9h]], [[2bni]], [[2cce]], [[2ccf]], [[2ccn]], [[2ahp]], [[2hy6]], [[3i1g]], [[2wpy]], [[2wpz]], [[2wq0]], [[2wq1]], [[2wq2]], [[2wq3]], [[3k7z]], [[3gjp]], [[3m48]] – yGcn4 (mutant)<br /> | + | **[[1gcl]], [[1swi]], [[1ce9]], [[1w5g]], [[1w5h]], [[1w5j]], [[1w5k]], [[1w5l]], [[1unt]], [[1unu]], [[1unv]], [[1unw]], [[1unx]], [[1uny]], [[1unz]], [[1uo0]], [[1uo1]], [[1uo2]], [[1uo3]], [[1uo4]], [[1uo5]], [[1u9f]], [[1u9g]], [[1u9h]], [[2bni]], [[2cce]], [[2ccf]], [[2ccn]], [[2ahp]], [[2hy6]], [[3i1g]], [[2wpy]], [[2wpz]], [[2wq0]], [[2wq1]], [[2wq2]], [[2wq3]], [[3k7z]], [[3gjp]], [[3m48]], [[4niz]], [[4nj0]], [[4nj1]], [[4nj2]], [[4tl1]] – yGcn4 (mutant)<br /> |
**[[1zii]], [[1zik]], [[1zil]] – yGcn4 dimer (mutant)<br /> | **[[1zii]], [[1zik]], [[1zil]] – yGcn4 dimer (mutant)<br /> | ||
**[[2o7h]], [[1gcm]], [[1zij]], [[1zim]], [[1piq]], [[1ij0]], [[1ij1]], [[1ij2]], [[1ij3]], [[1rb4]], [[1rb5]], [[1rb6]] – yGcn4 trimer (mutant)<br /> | **[[2o7h]], [[1gcm]], [[1zij]], [[1zim]], [[1piq]], [[1ij0]], [[1ij1]], [[1ij2]], [[1ij3]], [[1rb4]], [[1rb5]], [[1rb6]] – yGcn4 trimer (mutant)<br /> | ||
**[[2nrn]] – yGcn4 tetramer (mutant)<br /> | **[[2nrn]] – yGcn4 tetramer (mutant)<br /> | ||
**[[1fmh]] – yGcn4 dimer (mutant) - NMR<br /> | **[[1fmh]] – yGcn4 dimer (mutant) - NMR<br /> | ||
| - | **[[1c94]] – yGcn4 retro<br /> | ||
**[[1ysa]], [[1dgc]], [[2dgc]] – yGcn4 dimer + DNA <br /> | **[[1ysa]], [[1dgc]], [[2dgc]] – yGcn4 dimer + DNA <br /> | ||
| + | |||
| + | *Gcn4-pLI | ||
| + | |||
| + | **[[2ag3]], [[2oxj]], [[2oxk]] – yGcn4-pLI (br /> | ||
| + | **[[1u2u]] – yGcn4-pLI dimer - NMR<br /> | ||
| + | **[[4dmd]], [[1kd8]], [[1kd9]], [[1kdd]] – yGcn4-pLI dimer <br /> | ||
| + | **[[4dme]] – yGcn4-pLI trimer <br /> | ||
| + | **[[1c94]] – yGcn4-pLI retro<br /> | ||
| + | |||
| + | *Gcn4 transcription activation domain | ||
| + | |||
**[[2lpb]] – yGcn4 + mediator of RNA polymerase II<br /> | **[[2lpb]] – yGcn4 + mediator of RNA polymerase II<br /> | ||
Revision as of 09:51, 6 April 2015
Gcn4 is a yeast transcriptional activator. It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a DNA recognition motif consisting of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induces the transition of this region from unstructured to α-helical. Gcn4-pLI is a 33-residue peptide of the dimerization domain of Gcn4. See also Intrinsically Disordered Protein.
3D Structures of Gcn4
Updated on 06-April-2015
