1lvr
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1lvq|1LVQ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvr OCA], [http://www.ebi.ac.uk/pdbsum/1lvr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lvr RCSB]</span> | ||
}} | }} | ||
| Line 32: | Line 35: | ||
[[Category: transferred no]] | [[Category: transferred no]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:58 2008'' |
Revision as of 19:08, 30 March 2008
| |||||||
| Related: | 1LVQ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Overview
The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
About this Structure
1LVR is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474
Page seeded by OCA on Sun Mar 30 22:07:58 2008
