4rle
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rle RCSB], [http://www.ebi.ac.uk/pdbsum/4rle PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rle RCSB], [http://www.ebi.ac.uk/pdbsum/4rle PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The cyclic dimeric AMP nucleotide c-di-AMP is an essential second messenger in Bacillus subtilis. We have identified the protein DarA as one of the prominent c-di-AMP receptors in B. subtilis. Crystal structure analysis shows that DarA is highly homologous to PII signal transducer proteins. In contrast to PII proteins the functionally important B and T loops are swapped with respect to their size. DarA is a homo-trimer that binds three molecules of c-di-AMP, each in a pocket located between two subunits. We demonstrate that DarA is capable to bind c-di-AMP and, with lower affinity, also 3'3'-cGAMP, but not c-di-GMP or 2'3'-cGAMP. Consistently the crystal structure shows that within the ligand-binding pocket only one adenine is highly specifically recognized, while the pocket for the other adenine appears to be promiscuous. Comparison with a homologous ligand-free DarA structure reveals that c-di-AMP binding is accompanied by conformational changes of both the fold and the position of the B loop in DarA. | ||
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| + | Identification, characterization and structure analysis of the c-di-AMP binding PII-like signal transduction protein DarA.,Gundlach J, Dickmanns A, Schroder-Tittmann K, Neumann P, Kaesler J, Kampf J, Herzberg C, Hammer E, Schwede F, Kaever V, Tittmann K, Stulke J, Ficner R J Biol Chem. 2014 Nov 28. pii: jbc.M114.619619. PMID:25433025<ref>PMID:25433025</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 09:53, 17 December 2014
Crystal structure of the c-di-AMP binding PII-like protein DarA
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