1mdw
From Proteopedia
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|PDB= 1mdw |SIZE=350|CAPTION= <scene name='initialview01'>1mdw</scene>, resolution 1.95Å | |PDB= 1mdw |SIZE=350|CAPTION= <scene name='initialview01'>1mdw</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 and 3.4.22.53 3.4.22.52 and 3.4.22.53] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 and 3.4.22.53 3.4.22.52 and 3.4.22.53] </span> |
|GENE= CALPAIN II ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |GENE= CALPAIN II ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kxr|1KXR]], [[1kfu|1KFU]], [[1kfx|1KFX]], [[1dfo|1DFO]], [[1aj5|1AJ5]], [[1alw|1ALW]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mdw OCA], [http://www.ebi.ac.uk/pdbsum/1mdw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mdw RCSB]</span> | ||
}} | }} | ||
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[[Category: Lim, D.]] | [[Category: Lim, D.]] | ||
[[Category: Moldoveanu, T.]] | [[Category: Moldoveanu, T.]] | ||
| - | [[Category: CA]] | ||
[[Category: calpain cysteine protease fold]] | [[Category: calpain cysteine protease fold]] | ||
[[Category: helix instability]] | [[Category: helix instability]] | ||
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[[Category: two cooperative calcium site]] | [[Category: two cooperative calcium site]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:14:51 2008'' |
Revision as of 19:14, 30 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | CALPAIN II (Rattus norvegicus) | ||||||
| Activity: | Hydrolase, with EC number and 3.4.22.53 3.4.22.52 and 3.4.22.53 | ||||||
| Related: | 1KXR, 1KFU, 1KFX, 1DFO, 1AJ5, 1ALW
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Calcium-Bound Protease Core of Calpain II Reveals the Basis for Intrinsic Inactivation
Overview
Uncontrolled activation of calpain can lead to necrotic cell death and irreversible tissue damage. We have discovered an intrinsic mechanism whereby the autolysis-generated protease core fragment of calpain is inactivated through the inherent instability of a key alpha-helix. This auto-inactivation state was captured by the 1.9 A Ca(2+)-bound structure of the protease core from m-calpain, and sequence alignments suggest that it applies to about half of the calpain isoforms. Intact calpain large subunits are also subject to this inhibition, which can be prevented through assembly of the heterodimers. Other isoforms or their released cores are not silenced by this mechanism and might contribute to calpain patho-physiologies.
About this Structure
1MDW is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Calpain silencing by a reversible intrinsic mechanism., Moldoveanu T, Hosfield CM, Lim D, Jia Z, Davies PL, Nat Struct Biol. 2003 May;10(5):371-8. PMID:12665854
Page seeded by OCA on Sun Mar 30 22:14:51 2008
