1mey
From Proteopedia
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|PDB= 1mey |SIZE=350|CAPTION= <scene name='initialview01'>1mey</scene>, resolution 2.200Å | |PDB= 1mey |SIZE=350|CAPTION= <scene name='initialview01'>1mey</scene>, resolution 2.200Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=C38:5-IODO-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE'>C38</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mey OCA], [http://www.ebi.ac.uk/pdbsum/1mey PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mey RCSB]</span> | ||
}} | }} | ||
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[[Category: Berg, J M.]] | [[Category: Berg, J M.]] | ||
[[Category: Kim, C A.]] | [[Category: Kim, C A.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: ZN]] | ||
[[Category: complex (zinc finger/dna)]] | [[Category: complex (zinc finger/dna)]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:15:17 2008'' |
Revision as of 19:15, 30 March 2008
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| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA
Overview
Considerable recent effort has been devoted to the design and selection of sequence-specific DNA binding proteins based on tandem arrays of Cys2His2 zinc finger domains. While the DNA binding properties of these designed proteins have been studied extensively, the structural basis for site-specific binding has not been examined experimentally. Here we report the crystal structure of a complex between a protein comprised of three consensus-sequence-based zinc finger domains and an oligonucleotide corresponding to a favourable DNA binding site. This structure reveals relatively simple modular interactions and structural adaptations that compensate for differences in contact residue side-chain lengths.
About this Structure
1MEY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A 2.2 A resolution crystal structure of a designed zinc finger protein bound to DNA., Kim CA, Berg JM, Nat Struct Biol. 1996 Nov;3(11):940-5. PMID:8901872 [[Category: ]]
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