5fit
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The histidine triad (HIT) protein family is among the most ubiquitous and, highly conserved in nature, but a biological activity has not yet been, identified for any member of the HIT family. Fragile histidine triad, protein (FHIT) and protein kinase C interacting protein (PKCI) were used, in a structure-based approach to elucidate characteristics of in vivo, ligands and reactions. Crystallographic structures of apo, substrate, analog, pentacovalent transition-state analog, and product states of both, enzymes reveal a catalytic mechanism and define substrate characteristics, required for catalysis, thus unifying the HIT family as nucleotidyl, hydrolases, transferases, or both. The approach described here may be, useful in identifying structure-function relations between protein, families . | + | The histidine triad (HIT) protein family is among the most ubiquitous and, highly conserved in nature, but a biological activity has not yet been, identified for any member of the HIT family. Fragile histidine triad, protein (FHIT) and protein kinase C interacting protein (PKCI) were used, in a structure-based approach to elucidate characteristics of in vivo, ligands and reactions. Crystallographic structures of apo, substrate, analog, pentacovalent transition-state analog, and product states of both, enzymes reveal a catalytic mechanism and define substrate characteristics, required for catalysis, thus unifying the HIT family as nucleotidyl, hydrolases, transferases, or both. The approach described here may be, useful in identifying structure-function relations between protein, families identified through genomics. |
==About this Structure== | ==About this Structure== | ||
| - | 5FIT is a | + | 5FIT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with AP2 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Bis(5'-adenosyl)-triphosphatase Bis(5'-adenosyl)-triphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.29 3.6.1.29] Structure known Active Sites: AVE and HNE. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5FIT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: putative tumor suppressor]] | [[Category: putative tumor suppressor]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:35:59 2007'' |
Revision as of 13:30, 5 November 2007
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FHIT-SUBSTRATE ANALOG
Overview
The histidine triad (HIT) protein family is among the most ubiquitous and, highly conserved in nature, but a biological activity has not yet been, identified for any member of the HIT family. Fragile histidine triad, protein (FHIT) and protein kinase C interacting protein (PKCI) were used, in a structure-based approach to elucidate characteristics of in vivo, ligands and reactions. Crystallographic structures of apo, substrate, analog, pentacovalent transition-state analog, and product states of both, enzymes reveal a catalytic mechanism and define substrate characteristics, required for catalysis, thus unifying the HIT family as nucleotidyl, hydrolases, transferases, or both. The approach described here may be, useful in identifying structure-function relations between protein, families identified through genomics.
About this Structure
5FIT is a Single protein structure of sequence from Homo sapiens with AP2 as ligand. Active as Bis(5'-adenosyl)-triphosphatase, with EC number 3.6.1.29 Structure known Active Sites: AVE and HNE. Full crystallographic information is available from OCA.
Reference
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207
Page seeded by OCA on Mon Nov 5 15:35:59 2007
Categories: Bis(5'-adenosyl)-triphosphatase | Homo sapiens | Single protein | Hendrickson, W.A. | Klein, M.G. | Lima, C.D. | AP2 | Fhit | Fragile histidine triad protein | Histidine triad protein family | Hit protein family | Hydrolase | Nucleotidyl hydrolase | Nucleotidyl transferase | Putative tumor suppressor
