1mi6
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1gqe|1GQE]], [[1dt9|1DT9]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mi6 OCA], [http://www.ebi.ac.uk/pdbsum/1mi6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mi6 RCSB]</span> | ||
}} | }} | ||
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[[Category: Zavialov, A V.]] | [[Category: Zavialov, A V.]] | ||
[[Category: conformational change]] | [[Category: conformational change]] | ||
| - | [[Category: release complex | + | [[Category: rf2,release complex]] |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:21 2008'' |
Revision as of 19:16, 30 March 2008
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| Related: | 1GQE, 1DT9
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Docking of the modified RF2 X-ray structure into the Low Resolution Cryo-EM map of RF2 E.coli 70S Ribosome
Overview
Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC.
About this Structure
1MI6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A cryo-electron microscopic study of ribosome-bound termination factor RF2., Rawat UB, Zavialov AV, Sengupta J, Valle M, Grassucci RA, Linde J, Vestergaard B, Ehrenberg M, Frank J, Nature. 2003 Jan 2;421(6918):87-90. PMID:12511960
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