Protein kinase Spk1

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'''Protein kinase Spk1 (Rad53)''' is a serine/threonine protein kinase which phosphorylates proteins. Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints. Rad53 phosphorylates proteins at serine, threonine and tyrosine residues.
'''Protein kinase Spk1 (Rad53)''' is a serine/threonine protein kinase which phosphorylates proteins. Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints. Rad53 phosphorylates proteins at serine, threonine and tyrosine residues.
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== Disease ==
 
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== Relevance ==
 
== Structural highlights ==
== Structural highlights ==
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Rad53 contains phosphothreonine recognition domains: '''FHA1''' at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to phosphothreonine and '''FHA2''' at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to phosphothreonine. Two '''SCD''' - SQ/TQ-rich cluster domains – are flanking the '''kinase''' domain.
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Rad53 contains phosphothreonine recognition domains: '''FHA1''' at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to phosphothreonine and '''FHA2''' at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to phosphothreonine. Two '''SCD''' - SQ/TQ-rich cluster domains – are flanking the '''kinase''' domain. The yeast FHA1 domain interacts with peptide containing phosphothreonine<ref>PMID:11106755</ref>.
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Revision as of 10:30, 14 July 2016

Structure of yeast Rad53 FHA1 domain (grey) complex with phosphothreonine peptide (green) (PDB code 2a0t).

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3D structures of protein kinase Spk1

Updated on 14-July-2016

References

  1. Durocher D, Taylor IA, Sarbassova D, Haire LF, Westcott SL, Jackson SP, Smerdon SJ, Yaffe MB. The molecular basis of FHA domain:phosphopeptide binding specificity and implications for phospho-dependent signaling mechanisms. Mol Cell. 2000 Nov;6(5):1169-82. PMID:11106755

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