1msw
From Proteopedia
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|PDB= 1msw |SIZE=350|CAPTION= <scene name='initialview01'>1msw</scene>, resolution 2.10Å | |PDB= 1msw |SIZE=350|CAPTION= <scene name='initialview01'>1msw</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1msw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msw OCA], [http://www.ebi.ac.uk/pdbsum/1msw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1msw RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MSW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1MSW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. The following page contains interesting information on the relation of 1MSW with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb40_1.html RNA Polymerase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSW OCA]. |
==Reference== | ==Reference== | ||
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase., Yin YW, Steitz TA, Science. 2002 Nov 15;298(5597):1387-95. Epub 2002 Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12242451 12242451] | Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase., Yin YW, Steitz TA, Science. 2002 Nov 15;298(5597):1387-95. Epub 2002 Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12242451 12242451] | ||
| - | [[Category: Bacteriophage t7]] | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
| + | [[Category: Enterobacteria phage t7]] | ||
[[Category: RNA Polymerase]] | [[Category: RNA Polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: t7rnap elongation complex]] | [[Category: t7rnap elongation complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:20:41 2008'' |
Revision as of 19:20, 30 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase
Overview
To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2.1 angstrom resolution the crystal structure of a T7 RNAP elongation complex with 30 base pairs of duplex DNA containing a "transcription bubble" interacting with a 17-nucleotide RNA transcript. The transition from an initiation to an elongation complex is accompanied by a major refolding of the amino-terminal 300 residues. This results in loss of the promoter binding site, facilitating promoter clearance, and creates a tunnel that surrounds the RNA transcript after it peels off a seven-base pair heteroduplex. Formation of the exit tunnel explains the enhanced processivity of the elongation complex. Downstream duplex DNA binds to the fingers domain, and its orientation relative to upstream DNA in the initiation complex implies an unwinding that could facilitate formation of the open promoter complex.
About this Structure
1MSW is a Single protein structure of sequence from Enterobacteria phage t7. The following page contains interesting information on the relation of 1MSW with [RNA Polymerase]. Full crystallographic information is available from OCA.
Reference
Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase., Yin YW, Steitz TA, Science. 2002 Nov 15;298(5597):1387-95. Epub 2002 Sep 19. PMID:12242451
Page seeded by OCA on Sun Mar 30 22:20:41 2008
