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1mur

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|PDB= 1mur |SIZE=350|CAPTION= <scene name='initialview01'>1mur</scene>, resolution 2.50&Aring;
|PDB= 1mur |SIZE=350|CAPTION= <scene name='initialview01'>1mur</scene>, resolution 2.50&Aring;
|SITE=
|SITE=
-
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
 +
|DOMAIN=
 +
|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mur OCA], [http://www.ebi.ac.uk/pdbsum/1mur PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mur RCSB]</span>
}}
}}
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[[Category: Reznikoff, W R.]]
[[Category: Reznikoff, W R.]]
[[Category: Thoden, J B.]]
[[Category: Thoden, J B.]]
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[[Category: MN]]
 
[[Category: dna binding]]
[[Category: dna binding]]
[[Category: transposase]]
[[Category: transposase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:48:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:21:23 2008''

Revision as of 19:21, 30 March 2008

Image:1mur.gif


PDB ID 1mur

Drag the structure with the mouse to rotate
, resolution 2.50Å
Ligands: , , , ,
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



TN5 TRANSPOSASE: 20MER OUTSIDE END 2 MN COMPLEX


Overview

A synaptic complex of Tn5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis.

About this Structure

1MUR is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1L1A. Full crystallographic information is available from OCA.

Reference

Two-metal active site binding of a Tn5 transposase synaptic complex., Lovell S, Goryshin IY, Reznikoff WR, Rayment I, Nat Struct Biol. 2002 Apr;9(4):278-81. PMID:11896402

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