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| - | ==Recognition of the amber stop codon by release factor RF1. This entry 3MR8 contains 30S ribosomal subunit. The 50S ribosomal subunit can be found in PDB entry 3MS1. Molecule B in the same asymmetric unit is deposited as 3MRZ (50S) and 3MS0 (30S).==
| + | #REDIRECT [[4v7p]] This PDB entry is obsolete and replaced by 4v7p |
| - | <StructureSection load='3mr8' size='340' side='right' caption='[[3mr8]], [[Resolution|resolution]] 3.62Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[3mr8]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MR8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MR8 FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f1e|3f1e]], [[3d5a|3d5a]], [[1zbt|1zbt]], [[3ms1|3ms1]], [[3ms0|3ms0]], [[3mrz|3mrz]]</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mr8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mr8 RCSB], [http://www.ebi.ac.uk/pdbsum/3mr8 PDBsum]</span></td></tr>
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| - | </table>
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| - | == Evolutionary Conservation ==
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| - | [[Image:Consurf_key_small.gif|200px|right]]
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| - | Check<jmol>
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| - | <jmolCheckbox>
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| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mr/3mr8_consurf.spt"</scriptWhenChecked>
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| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| - | <text>to colour the structure by Evolutionary Conservation</text>
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| - | </jmolCheckbox>
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| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| - | <div style="clear:both"></div>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | We report the crystal structure of a termination complex containing release factor RF1 bound to the 70S ribosome in response to an amber (UAG) codon at 3.6-A resolution. The amber codon is recognized in the 30S subunit-decoding centre directly by conserved elements of domain 2 of RF1, including T186 of the PVT motif. Together with earlier structures, the mechanisms of recognition of all three stop codons by release factors RF1 and RF2 can now be described. Our structure confirms that the backbone amide of Q230 of the universally conserved GGQ motif is positioned to contribute directly to the catalysis of the peptidyl-tRNA hydrolysis reaction through stabilization of the leaving group and/or transition state. We also observe synthetic-negative interactions between mutations in the switch loop of RF1 and in helix 69 of 23S rRNA, revealing that these structural features interact functionally in the termination process. These findings are consistent with our proposal that structural rearrangements of RF1 and RF2 are critical to accurate translation termination.
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| - | Recognition of the amber UAG stop codon by release factor RF1.,Korostelev A, Zhu J, Asahara H, Noller HF EMBO J. 2010 Aug 4;29(15):2577-85. Epub 2010 Jun 29. PMID:20588254<ref>PMID:20588254</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Ribosomal protein THX|Ribosomal protein THX]]
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| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Asahara, H]]
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| - | [[Category: Korostelev, A]]
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| - | [[Category: Noller, H F]]
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| - | [[Category: Zhu, J]]
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| - | [[Category: Amber]]
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| - | [[Category: Release factor]]
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| - | [[Category: Rf1]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Stop codon]]
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| - | [[Category: Termination]]
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| - | [[Category: Uag]]
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