1nb7
From Proteopedia
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|PDB= 1nb7 |SIZE=350|CAPTION= <scene name='initialview01'>1nb7</scene>, resolution 2.9Å | |PDB= 1nb7 |SIZE=350|CAPTION= <scene name='initialview01'>1nb7</scene>, resolution 2.9Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1nb4|1NB4]], [[1nb6|1NB6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nb7 OCA], [http://www.ebi.ac.uk/pdbsum/1nb7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nb7 RCSB]</span> | ||
}} | }} | ||
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[[Category: Rowlands, D J.]] | [[Category: Rowlands, D J.]] | ||
[[Category: Trowbridge, R.]] | [[Category: Trowbridge, R.]] | ||
| - | [[Category: MN]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: de-novo priming]] | [[Category: de-novo priming]] | ||
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[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:27:55 2008'' |
Revision as of 19:27, 30 March 2008
| |||||||
| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | RNA-directed RNA polymerase, with EC number 2.7.7.48 | ||||||
| Related: | 1NB4, 1NB6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HC-J4 RNA polymerase complexed with short RNA template strand
Overview
Several crystal structures of the hepatitis C virus NS5B protein (genotype-1b, strain J4) complexed with metal ions, single-stranded RNA or nucleoside-triphosphates have been determined. These complexes illustrate how conserved amino acid side-chains, together with essential structural features within the active site, control nucleotide binding and likely mediate de-novo initiation. The incoming nucleotide interacts with several basic residues from an extension on the NS5B fingers domain, a beta-hairpin from the NS5B thumb domain and the C-terminal arm. The modular, bi-partite fingers domain carries a long binding groove which guides the template towards the catalytic site. The apo-polymerase structure provides unprecedented insights into potential non-nucleoside inhibitor binding sites located between palm and thumb near motif E, which is unique to RNA polymerases and reverse transcriptases.
About this Structure
1NB7 is a Single protein structure of sequence from Hepatitis c virus. Full crystallographic information is available from OCA.
Reference
Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): structural evidence for nucleotide import and de-novo initiation., O'Farrell D, Trowbridge R, Rowlands D, Jager J, J Mol Biol. 2003 Feb 28;326(4):1025-35. PMID:12589751
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