1nco
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1nco |SIZE=350|CAPTION= <scene name='initialview01'>1nco</scene>, resolution 1.8Å | |PDB= 1nco |SIZE=350|CAPTION= <scene name='initialview01'>1nco</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CHR:NEOCARZINOSTATIN-CHROMOPHORE'>CHR</scene> | + | |LIGAND= <scene name='pdbligand=CHR:NEOCARZINOSTATIN-CHROMOPHORE'>CHR</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nco OCA], [http://www.ebi.ac.uk/pdbsum/1nco PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nco RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Myers, A G.]] | [[Category: Myers, A G.]] | ||
[[Category: Rees, D C.]] | [[Category: Rees, D C.]] | ||
| - | [[Category: CHR]] | ||
| - | [[Category: MRD]] | ||
[[Category: antibacterial and antitumor protein]] | [[Category: antibacterial and antitumor protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:28:32 2008'' |
Revision as of 19:28, 30 March 2008
| |||||||
| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN
Overview
Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. The chromophore pi-face interacts with the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition during chromophore activation, are unexposed. The position of the amino group of the chromophore carbohydrate relative to C-12 supports the idea that the amino group plays a role in thiol activation.
About this Structure
1NCO is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:8235619
Page seeded by OCA on Sun Mar 30 22:28:32 2008
