1nhl
From Proteopedia
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|PDB= 1nhl |SIZE=350|CAPTION= <scene name='initialview01'>1nhl</scene>, resolution 2.3Å | |PDB= 1nhl |SIZE=350|CAPTION= <scene name='initialview01'>1nhl</scene>, resolution 2.3Å | ||
|SITE= | |SITE= | ||
- | |LIGAND= | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SNAP23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= SNAP23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
+ | |DOMAIN= | ||
+ | |RELATEDENTRY=[[1sfc|1SFC]] | ||
+ | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhl OCA], [http://www.ebi.ac.uk/pdbsum/1nhl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nhl RCSB]</span> | ||
}} | }} | ||
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[[Category: snare]] | [[Category: snare]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:30:30 2008'' |
Revision as of 19:30, 30 March 2008
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, resolution 2.3Å | |||||||
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Ligands: | |||||||
Gene: | SNAP23 (Homo sapiens) | ||||||
Related: | 1SFC
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Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
Coordinates: | save as pdb, mmCIF, xml |
SNAP-23N Structure
Overview
SNARE proteins mediate intracellular membrane fusion by forming a coiled-coil complex to merge opposing membranes. A "fusion-active" neuronal SNARE complex is a parallel four-helix bundle containing two coiled-coil domains from SNAP-25 and one coiled-coil domain each from syntaxin-1a and VAMP-2. "Prefusion" assembly intermediate complexes can also form from these SNAREs. We studied the N-terminal coiled-coil domain of SNAP-23 (SNAP-23N), a non-neuronal homologue of SNAP-25, and its interaction with other coiled-coil domains. SNAP-23N can assemble spontaneously with the coiled-coil domains from SNAP-23C, syntaxin-4, and VAMP-3 to form a heterotetrameric complex. Unexpectedly, pure SNAP-23N crystallizes as a coiled-coil homotetrameric complex. The four helices have a parallel orientation and are symmetrical about the long axis. The complex is stabilized through the interaction of conserved hydrophobic residues comprising the a and d positions of the coiled-coil heptad repeats. In addition, a central, highly conserved glutamine residue (Gln-48) is buried within the interface by hydrogen bonding between glutamine side chains derived from adjacent subunits and to solvent molecules. A comparison of the SNAP-23N structure to other SNARE complex structures reveals how a simple coiled-coil motif can form diverse SNARE complexes.
About this Structure
1NHL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:12556468
Page seeded by OCA on Sun Mar 30 22:30:30 2008
Categories: Homo sapiens | Single protein | Eck, M J. | Freedman, S J. | Song, H K. | Xu, Y. | Coiled-coil | Snare