1njn
From Proteopedia
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|PDB= 1njn |SIZE=350|CAPTION= <scene name='initialview01'>1njn</scene>, resolution 3.70Å | |PDB= 1njn |SIZE=350|CAPTION= <scene name='initialview01'>1njn</scene>, resolution 3.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SPS:SPARSOMYCIN'>SPS</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SPS:SPARSOMYCIN'>SPS</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1njm|1NJM]], [[1njo|1NJO]], [[1njp|1NJP]], [[1k01|1K01]], [[1jzx|1JZX]], [[1jzy|1JZY]], [[1jzz|1JZZ]], [[1k00|1K00]], [[1nkw|1NKW]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1njn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njn OCA], [http://www.ebi.ac.uk/pdbsum/1njn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1njn RCSB]</span> | ||
}} | }} | ||
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[[Category: Yonath, A.]] | [[Category: Yonath, A.]] | ||
[[Category: Zarivatch, R.]] | [[Category: Zarivatch, R.]] | ||
| - | [[Category: SPS]] | ||
[[Category: peptide bond formation]] | [[Category: peptide bond formation]] | ||
[[Category: peptidyl-transferase]] | [[Category: peptidyl-transferase]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:16 2008'' |
Revision as of 19:31, 30 March 2008
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| , resolution 3.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Related: | 1NJM, 1NJO, 1NJP, 1K01, 1JZX, 1JZY, 1JZZ, 1K00, 1NKW
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with the antibiotic sparsomycin
Overview
Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.
About this Structure
1NJN is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.
Reference
Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression., Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A, Mol Cell. 2003 Jan;11(1):91-102. PMID:12535524
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