1not
From Proteopedia
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|PDB= 1not |SIZE=350|CAPTION= <scene name='initialview01'>1not</scene>, resolution 1.2Å | |PDB= 1not |SIZE=350|CAPTION= <scene name='initialview01'>1not</scene>, resolution 1.2Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene> | + | |LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1not FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1not OCA], [http://www.ebi.ac.uk/pdbsum/1not PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1not RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Martin, J L.]] | [[Category: Martin, J L.]] | ||
[[Category: Shan, L.]] | [[Category: Shan, L.]] | ||
| - | [[Category: NH2]] | ||
[[Category: acetylcholine receptor]] | [[Category: acetylcholine receptor]] | ||
[[Category: conotoxin]] | [[Category: conotoxin]] | ||
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[[Category: venom]] | [[Category: venom]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:33:19 2008'' |
Revision as of 19:33, 30 March 2008
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| , resolution 1.2Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE 1.2 ANGSTROM STRUCTURE OF G1 ALPHA CONOTOXIN
Overview
Predatory marine snails of the genus Conus paralyze their fish prey by injecting a potent toxin. The alpha-conotoxin GI is a 13-residue peptide isolated from venom of Conus geographus. It functions by blocking the postsynaptic nicotinic acetylcholine receptor. After crystallization in deionized water, the three-dimensional structure of the GI neurotoxin was determined to 1.2 A resolution by X-ray crystallography. This structure, which can be described as a triangular slab, shows overall similarities to those derived by NMR, CD, and predictive methods. The principal framework of the molecule is provided by two disulfide bonds, one linking Cys 2 and Cys 7 and the other Cys 3 and Cys 13. Opposite ends of the sequence are drawn together even further by hydrogen bonds between Glu 1 and Cys 13 and between Cys 2 and Ser 12. Since the C-terminus is amidated, only one negative charge is present (carboxylate of Glu 1), and this is not implicated in receptor binding. Two positively charged regions (the alpha-amino group of Glu 1 and the guanido group of Arg 9) are situated 15 A apart at the corners of the triangular face of the molecule. phi, psi angles characteristic of a 3(10) helix were observed for residues 5-7. For residues 8-11, these angles were consistent with either a type I beta-turn or a distorted 3(10) helix.
About this Structure
1NOT is a Single protein structure of sequence from Conus geographus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the alpha-conotoxin GI at 1.2 A resolution., Guddat LW, Martin JA, Shan L, Edmundson AB, Gray WR, Biochemistry. 1996 Sep 3;35(35):11329-35. PMID:8784187
Page seeded by OCA on Sun Mar 30 22:33:19 2008
