1nso
From Proteopedia
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= PRT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= PRT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11942 Simian retrovirus 1]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nso OCA], [http://www.ebi.ac.uk/pdbsum/1nso PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nso RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1NSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1NSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_retrovirus_1 Simian retrovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSO OCA]. |
==Reference== | ==Reference== | ||
Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14568536 14568536] | Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14568536 14568536] | ||
| - | [[Category: Simian retrovirus | + | [[Category: Simian retrovirus 1]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bauerova, H.]] | [[Category: Bauerova, H.]] | ||
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[[Category: virus maturation]] | [[Category: virus maturation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:34:56 2008'' |
Revision as of 19:34, 30 March 2008
| |||||||
| Gene: | PRT (Simian retrovirus 1) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Folded monomer of protease from Mason-Pfizer monkey virus
Overview
The assembly of Mason-Pfizer monkey virus Gag polyproteins into immature capsids and their cleavage by the encoded protease are temporally and spatially separated processes, making the virus a particularly useful model for investigation of protease activation. Here we present a high resolution NMR structure of a fully folded monomer of a 12 kDa M-PMV protease (wt 12 PR) and of a Cys7Ala/Asp26Asn/Cys106Ala mutant (12 PR(D26N/C7A/C106A)). The overall structures of both wt 12 PR and 12 PR(D26N/C7A/C106A) follow the conservative structural motif of other retroviral proteases. The most prominent difference from the canonical fold of retroviral proteases is the absence of the interfacial beta-sheet, which leads to the loss of the principal force stabilizing the dimer of M-PMV PR. The monomer-dimer equilibrium can be shifted in favor of the dimer by adding a substrate or an inhibitor, partially compensating for the missing role of the beta-sheet. We also show that cysteines C7 and C106 play a crucial role in stabilizing the dimer and consequently increasing the proteolytic activity of M-PMV PR. This is consistent with the role of reversible oxidative modification of the cysteine residues in the regulation of the maturation of assembled M-PMV capsids in the cytoplasm.
About this Structure
1NSO is a Single protein structure of sequence from Simian retrovirus 1. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:14568536
Page seeded by OCA on Sun Mar 30 22:34:56 2008
