4dgw

From Proteopedia

(Difference between revisions)

Revision as of 02:50, 11 December 2016

Crystal Structure of the SF3a splicing factor complex of U2 snRNP

Structural highlights

4dgw is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PRP9_YEAST] mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly. [PRP11_YEAST] mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.^[1] [PRP21_YEAST] mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.

Publication Abstract from PubMed

SF3a is an evolutionarily conserved heterotrimeric complex essential for pre-mRNA splicing. It functions in spliceosome assembly within the mature U2 snRNP (small nuclear ribonucleoprotein particle), and its displacement from the spliceosome initiates the first step of the splicing reaction. We have identified a core domain of the yeast SF3a complex required for complex assembly and determined its crystal structure. The structure shows a bifurcated assembly of three subunits, Prp9, Prp11 and Prp21, with Prp9 interacting with Prp21 via a bidentate-binding mode, and Prp21 wrapping around Prp11. Structure-guided biochemical analysis also shows that Prp9 harbours a major binding site for stem-loop IIa of U2 snRNA. These findings provide mechanistic insights into the assembly of U2 snRNP.

Structure and assembly of the SF3a splicing factor complex of U2 snRNP.,Lin PC, Xu RM EMBO J. 2012 Feb 7;31(6):1579-90. doi: 10.1038/emboj.2012.7. PMID:22314233^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yan D, Perriman R, Igel H, Howe KJ, Neville M, Ares M Jr. CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif. Mol Cell Biol. 1998 Sep;18(9):5000-9. PMID:9710584
↑ Lin PC, Xu RM. Structure and assembly of the SF3a splicing factor complex of U2 snRNP. EMBO J. 2012 Feb 7;31(6):1579-90. doi: 10.1038/emboj.2012.7. PMID:22314233 doi:10.1038/emboj.2012.7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dgw"

Categories: Saccharomyces cerevisiae | Lin, P C | Splicing | Zinc finger

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the SF3a splicing factor complex of U2 snRNP==
 <StructureSection load='4dgw' size='340' side='right' caption='[[4dgw]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dgw RCSB], [http://www.ebi.ac.uk/pdbsum/4dgw PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dgw OCA], [http://pdbe.org/4dgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dgw RCSB], [http://www.ebi.ac.uk/pdbsum/4dgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dgw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/PRP9_YEAST PRP9_YEAST]] mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly. [[http://www.uniprot.org/uniprot/PRP11_YEAST PRP11_YEAST]] mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.<ref>PMID:9710584</ref>  [[http://www.uniprot.org/uniprot/PRP21_YEAST PRP21_YEAST]] mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4dgw" style="background-color:#fffaf0;"></div>
 ==See Also==

4dgw

From Proteopedia

Revision as of 02:50, 11 December 2016

Crystal Structure of the SF3a splicing factor complex of U2 snRNP

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox