1oio
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oio OCA], [http://www.ebi.ac.uk/pdbsum/1oio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oio RCSB]</span> | ||
}} | }} | ||
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[[Category: Tanskanen, J.]] | [[Category: Tanskanen, J.]] | ||
[[Category: Westerlund-Wikstrom, B.]] | [[Category: Westerlund-Wikstrom, B.]] | ||
| - | [[Category: NAG]] | ||
[[Category: adhesin]] | [[Category: adhesin]] | ||
[[Category: glcnac binding lectin]] | [[Category: glcnac binding lectin]] | ||
[[Category: n-acetyl-d-glucosamine binding]] | [[Category: n-acetyl-d-glucosamine binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:45:53 2008'' |
Revision as of 19:45, 30 March 2008
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| , resolution 1.70Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GAFD (F17C-TYPE) FIMBRIAL ADHESIN FROM ESCHERICHIA COLI
Overview
GafD in Escherichia coli G (F17) fimbriae is associated with diarrheal disease, and the structure of the ligand-binding domain, GafD1-178, has been determined at 1.7A resolution in the presence of the receptor sugar N-acetyl-D-glucosamine. The overall fold is a beta-barrel jelly-roll fold. The ligand-binding site was identified and localized to the side of the molecule. Receptor binding is mediated by side-chain as well main-chain interactions. Ala43-Asn44, Ser116-Thr117 form the sugar acetamide specificity pocket, while Asp88 confers tight binding and Trp109 appears to position the ligand. There is a disulfide bond that rigidifies the acetamide specificity pocket. The three fimbrial lectins, GafD, FimH and PapG share similar beta-barrel folds but display different ligand-binding regions and disulfide-bond patterns. We suggest an evolutionary path for the evolution of the very diverse fimbrial lectins from a common ancestral fold.
About this Structure
1OIO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structural basis of receptor-binding by Escherichia coli associated with diarrhea and septicemia., Merckel MC, Tanskanen J, Edelman S, Westerlund-Wikstrom B, Korhonen TK, Goldman A, J Mol Biol. 2003 Aug 22;331(4):897-905. PMID:12909017
Page seeded by OCA on Sun Mar 30 22:45:53 2008
