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1ovv

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Revision as of 19:51, 30 March 2008

Image:1ovv.jpg

, resolution 2.90Å

Ligands:

, ,

Related:

1ec5, 1jm0, 1jmb, 1lt1, 1OVR, 1OVU

Resources:

FirstGlance, OCA, PDBsum, RCSB

Coordinates:

save as pdb, mmCIF, xml

CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)

Overview

Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has been assigned using the group and minimal non-isomorphic supergroup relation between the space group of the previously determined di-Mn(II)-DF1-L13G structure and the space group of this form. This unconventional method of solving the phase problem has also been tested with form 2 using a reverse relation. The structure of the latter form has been solved using the group and maximal non-isomorphic subgroup relation with the space group of form 2 of the analogous dimanganese protein. This application has shown that this phasing method can be used for solving the protein structures of polymorphic crystals as an alternative to the molecular-replacement method.

About this Structure

1OVV is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:12876346

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