1owf
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1owf |SIZE=350|CAPTION= <scene name='initialview01'>1owf</scene>, resolution 1.95Å | |PDB= 1owf |SIZE=350|CAPTION= <scene name='initialview01'>1owf</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ihf|1IHF]], [[1ouz|1OUZ]], [[1owg|1OWG]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1owf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1owf OCA], [http://www.ebi.ac.uk/pdbsum/1owf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1owf RCSB]</span> | ||
}} | }} | ||
| Line 33: | Line 36: | ||
[[Category: protein-dna recognition]] | [[Category: protein-dna recognition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:19 2008'' |
Revision as of 19:51, 30 March 2008
| |||||||
| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Related: | 1IHF, 1OUZ, 1OWG
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site
Overview
Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.
About this Structure
1OWF is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466
Page seeded by OCA on Sun Mar 30 22:51:19 2008
