| Structural highlights
4n0h is a 3 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | |
| Related: | 4n0i |
| Gene: | GatA, GEP6, HER2, LRC6, YMR293C (Baker's yeast), GatB, PET112, YBL0724, YBL080C (Baker's yeast), GatF, GTF1, YGR102C (Baker's yeast) |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Function
[GATA_YEAST] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Required for HMG2-induced ER-remodeling.[1] [2] [GATF_YEAST] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Required for proper protein synthesis within the mitochondrion.[3] [4] [GATB_YEAST] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).[5]
Publication Abstract from PubMed
Yeast mitochondrial Gln-mtRNAGln is synthesized by the transamidation of mischarged Glu-mtRNAGln by a non-canonical heterotrimeric tRNA-dependent amidotransferase (AdT). The GatA and GatB subunits of the yeast AdT (GatFAB) are well conserved among bacteria and eukaryota, but the GatF subunit is a fungi-specific ortholog of the GatC subunit found in all other known heterotrimeric AdTs (GatCAB). Here we report the crystal structure of yeast mitochondrial GatFAB at 2.0 A resolution. The C-terminal region of GatF encircles the GatA-GatB interface in the same manner as GatC, but the N-terminal extension domain (NTD) of GatF forms several additional hydrophobic and hydrophilic interactions with GatA. NTD-deletion mutants displayed growth defects, but retained the ability to respire. Truncation of the NTD in purified mutants reduced glutaminase and transamidase activities when glutamine was used as the ammonia donor, but increased transamidase activity relative to the full-length enzyme when the donor was ammonium chloride. Our structure-based functional analyses suggest the NTD is a trans-acting scaffolding peptide for the GatA glutaminase active site. The positive surface charge and novel fold of the GatF-GatA interface, shown in this first crystal structure of an organellar AdT, stand in contrast with the more conventional, negatively charged bacterial AdTs described previously.
Crystal structure of Saccharomyces cerevisiae mitochondrial GatFAB reveals a novel subunit assembly in tRNA-dependent amidotransferases.,Araiso Y, Huot JL, Sekiguchi T, Frechin M, Fischer F, Enkler L, Senger B, Ishitani R, Becker HD, Nureki O Nucleic Acids Res. 2014 Apr 1. PMID:24692665[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Frechin M, Senger B, Braye M, Kern D, Martin RP, Becker HD. Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009 May 1;23(9):1119-30. doi: 10.1101/gad.518109. PMID:19417106 doi:http://dx.doi.org/10.1101/gad.518109
- ↑ Federovitch CM, Jones YZ, Tong AH, Boone C, Prinz WA, Hampton RY. Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae. Mol Biol Cell. 2008 Oct;19(10):4506-20. Epub 2008 Jul 30. PMID:18667535 doi:http://dx.doi.org/E07-11-1188
- ↑ Frechin M, Senger B, Braye M, Kern D, Martin RP, Becker HD. Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009 May 1;23(9):1119-30. doi: 10.1101/gad.518109. PMID:19417106 doi:http://dx.doi.org/10.1101/gad.518109
- ↑ Barros MH, Rak M, Paulela JA, Tzagoloff A. Characterization of Gtf1p, the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase. J Biol Chem. 2011 Sep 23;286(38):32937-47. doi: 10.1074/jbc.M111.265371. Epub, 2011 Jul 28. PMID:21799017 doi:http://dx.doi.org/10.1074/jbc.M111.265371
- ↑ Frechin M, Senger B, Braye M, Kern D, Martin RP, Becker HD. Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009 May 1;23(9):1119-30. doi: 10.1101/gad.518109. PMID:19417106 doi:http://dx.doi.org/10.1101/gad.518109
- ↑ Araiso Y, Huot JL, Sekiguchi T, Frechin M, Fischer F, Enkler L, Senger B, Ishitani R, Becker HD, Nureki O. Crystal structure of Saccharomyces cerevisiae mitochondrial GatFAB reveals a novel subunit assembly in tRNA-dependent amidotransferases. Nucleic Acids Res. 2014 Apr 1. PMID:24692665 doi:http://dx.doi.org/10.1093/nar/gku234
|
