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1oyu
From Proteopedia
| Line 5: | Line 5: | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[261l|261L]], [[262l|262L]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oyu OCA], [http://www.ebi.ac.uk/pdbsum/1oyu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oyu RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1OYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1OYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYU OCA]. |
==Reference== | ==Reference== | ||
Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12869697 12869697] | Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12869697 12869697] | ||
| - | [[Category: | + | [[Category: Enterobacteria phage t4]] |
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 31: | Line 34: | ||
[[Category: tandem repeat]] | [[Category: tandem repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:52:22 2008'' |
Revision as of 19:52, 30 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Related: | 261L, 262L
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Long-Distance conformational changes in a protein engineered by modulated sequence duplication
Overview
There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.
About this Structure
1OYU is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:12869697
Page seeded by OCA on Sun Mar 30 22:52:22 2008
