1p4n
From Proteopedia
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|PDB= 1p4n |SIZE=350|CAPTION= <scene name='initialview01'>1p4n</scene>, resolution 1.90Å | |PDB= 1p4n |SIZE=350|CAPTION= <scene name='initialview01'>1p4n</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UMA:URIDINE-5'-DIPHOSPHATE-N-ACETYLMURAMOYL-L-ALANINE'>UMA</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/UDP-N-acetylmuramoylpentapeptide-lysine_N(6)-alanyltransferase UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.10 2.3.2.10] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylmuramoylpentapeptide-lysine_N(6)-alanyltransferase UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.10 2.3.2.10] </span> |
|GENE= femx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1629 Weissella viridescens]) | |GENE= femx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1629 Weissella viridescens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ne9|1NE9]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4n OCA], [http://www.ebi.ac.uk/pdbsum/1p4n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p4n RCSB]</span> | ||
}} | }} | ||
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[[Category: Mayer, C.]] | [[Category: Mayer, C.]] | ||
[[Category: Sougakoff, W.]] | [[Category: Sougakoff, W.]] | ||
| - | + | [[Category: femx,ligase]] | |
| - | + | ||
| - | [[Category: femx | + | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:54:48 2008'' |
Revision as of 19:54, 30 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | femx (Weissella viridescens) | ||||||
| Activity: | UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase, with EC number 2.3.2.10 | ||||||
| Related: | 1NE9
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Weissella viridescens FemX:UDP-MurNAc-pentapeptide complex
Overview
Members of the FemABX protein family are novel therapeutic targets, as they are involved in the synthesis of the bacterial cell wall. They catalyze the addition of amino acid(s) on the peptidoglycan precursor using aminoacylated tRNA as a substrate. We report here the high-resolution structure of Weissella viridescens L-alanine transferase FemX and its complex with the UDP-MurNAc-pentapeptide. This is the first structure example of a FemABX family member that does not possess a coiled-coil domain. FemX consists of two structurally equivalent domains, separated by a cleft containing the binding site of the UDP-MurNAc-pentapeptide and a long channel that traverses one of the two domains. Our structural studies bring new insights into the evolution of the FemABX and the related GNAT superfamilies, shed light on the recognition site of the aminoacylated tRNA in Fem proteins, and allowed manual docking of the acceptor end of the alanyl-tRNAAla.
About this Structure
1P4N is a Single protein structure of sequence from Weissella viridescens. Full crystallographic information is available from OCA.
Reference
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition., Biarrotte-Sorin S, Maillard AP, Delettre J, Sougakoff W, Arthur M, Mayer C, Structure. 2004 Feb;12(2):257-67. PMID:14962386
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