1p9p
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | |LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/tRNA_(guanine-N(1)-)-methyltransferase tRNA (guanine-N(1)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.31 2.1.1.31] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_(guanine-N(1)-)-methyltransferase tRNA (guanine-N(1)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.31 2.1.1.31] </span> |
|GENE= TRMD OR B2607 OR C3128 OR Z3901 OR ECS3470 OR SF2667 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Escherichia coli, Escherichia coli O6, Escherichia coli O157:H7, and Shigella flexneri]) | |GENE= TRMD OR B2607 OR C3128 OR Z3901 OR ECS3470 OR SF2667 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Escherichia coli, Escherichia coli O6, Escherichia coli O157:H7, and Shigella flexneri]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9p OCA], [http://www.ebi.ac.uk/pdbsum/1p9p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p9p RCSB]</span> | ||
}} | }} | ||
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[[Category: Watts, J M.]] | [[Category: Watts, J M.]] | ||
[[Category: Zalacain, M.]] | [[Category: Zalacain, M.]] | ||
| - | [[Category: SAH]] | ||
[[Category: adomet]] | [[Category: adomet]] | ||
[[Category: knot]] | [[Category: knot]] | ||
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[[Category: spout]] | [[Category: spout]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:56:53 2008'' |
Revision as of 19:56, 30 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | TRMD OR B2607 OR C3128 OR Z3901 OR ECS3470 OR SF2667 (Escherichia coli, Escherichia coli O6, Escherichia coli O157:H7, and Shigella flexneri) | ||||||
| Activity: | tRNA (guanine-N(1)-)-methyltransferase, with EC number 2.1.1.31 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD
Overview
The crystal structure of Escherichia coli tRNA (guanosine-1) methyltransferase (TrmD) complexed with S-adenosyl homocysteine (AdoHcy) has been determined at 2.5A resolution. TrmD, which methylates G37 of tRNAs containing the sequence G36pG37, is a homo-dimer. Each monomer consists of a C-terminal domain connected by a flexible linker to an N-terminal AdoMet-binding domain. The two bound AdoHcy moieties are buried at the bottom of deep clefts. The dimer structure appears integral to the formation of the catalytic center of the enzyme and this arrangement strongly suggests that the anticodon loop of tRNA fits into one of these clefts for methyl transfer to occur. In addition, adjacent hydrophobic sites in the cleft delineate a defined pocket, which may accommodate the GpG sequence during catalysis. The dimer contains two deep trefoil peptide knots and a peptide loop extending from each knot embraces the AdoHcy adenine ring. Mutational analyses demonstrate that the knot is important for AdoMet binding and catalytic activity, and that the C-terminal domain is not only required for tRNA binding but plays a functional role in catalytic activity.
About this Structure
1P9P is a Single protein structure of sequence from Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri. Full crystallographic information is available from OCA.
Reference
Insights into catalysis by a knotted TrmD tRNA methyltransferase., Elkins PA, Watts JM, Zalacain M, van Thiel A, Vitazka PR, Redlak M, Andraos-Selim C, Rastinejad F, Holmes WM, J Mol Biol. 2003 Nov 7;333(5):931-49. PMID:14583191
Page seeded by OCA on Sun Mar 30 22:56:53 2008
Categories: Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri | Single protein | TRNA (guanine-N(1)-)-methyltransferase | Andraos-Selim, C. | Elkins, P A. | Holmes, W M. | Rastinejad, F. | Redlak, M. | Thiel, A Van. | Vitaszka, P R. | Watts, J M. | Zalacain, M. | Adomet | Knot | Methyltransferase | S-adenosylmethionine | Spou | Spout
