1q3o
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1q3o |SIZE=350|CAPTION= <scene name='initialview01'>1q3o</scene>, resolution 1.80Å | |PDB= 1q3o |SIZE=350|CAPTION= <scene name='initialview01'>1q3o</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene> | + | |LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= shank1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |GENE= shank1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1q3p|1Q3P]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q3o OCA], [http://www.ebi.ac.uk/pdbsum/1q3o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q3o RCSB]</span> | ||
}} | }} | ||
| Line 30: | Line 33: | ||
[[Category: Park, S J.]] | [[Category: Park, S J.]] | ||
[[Category: Rho, S H.]] | [[Category: Rho, S H.]] | ||
| - | [[Category: BR]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: gkap]] | [[Category: gkap]] | ||
| Line 36: | Line 38: | ||
[[Category: shank]] | [[Category: shank]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:08:22 2008'' |
Revision as of 20:08, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | shank1a (Rattus norvegicus) | ||||||
| Related: | 1Q3P
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Shank PDZ-ligand complex reveals a class I PDZ interaction and a novel PDZ-PDZ dimerization
Overview
The Shank/proline-rich synapse-associated protein family of multidomain proteins is known to play an important role in the organization of synaptic multiprotein complexes. For instance, the Shank PDZ domain binds to the C termini of guanylate kinase-associated proteins, which in turn interact with the guanylate kinase domain of postsynaptic density-95 scaffolding proteins. Here we describe the crystal structures of Shank1 PDZ in its peptide free form and in complex with the C-terminal hexapeptide (EAQTRL) of guanylate kinase-associated protein (GKAP1a) determined at 1.8- and 2.25-A resolutions, respectively. The structure shows the typical class I PDZ interaction of PDZ-peptide complex with the consensus sequence -X-(Thr/Ser)-X-Leu. In addition, Asp-634 within the Shank1 PDZ domain recognizes the positively charged Arg at -1 position and hydrogen bonds, and salt bridges between Arg-607 and the side chains of the ligand at -3 and -5 positions contribute further to the recognition of the peptide ligand. Remarkably, whether free or complexed, Shank1 PDZ domains form dimers with a conserved beta B/beta C loop and N-terminal beta A strands, suggesting a novel model of PDZ-PDZ homodimerization. This implies that antiparallel dimerization through the N-terminal beta A strands could be a common configuration among PDZ dimers. Within the dimeric structure, the two-peptide binding sites are arranged so that the N termini of the bound peptide ligands are in close proximity and oriented toward the 2-fold axis of the dimer. This configuration may provide a means of facilitating dimeric organization of PDZ-target assemblies.
About this Structure
1Q3O is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Shank PDZ-ligand complex reveals a class I PDZ interaction and a novel PDZ-PDZ dimerization., Im YJ, Lee JH, Park SH, Park SJ, Rho SH, Kang GB, Kim E, Eom SH, J Biol Chem. 2003 Nov 28;278(48):48099-104. Epub 2003 Sep 3. PMID:12954649
Page seeded by OCA on Sun Mar 30 23:08:22 2008
Categories: Rattus norvegicus | Single protein | Eom, S H. | Im, Y J. | Kang, G B. | Kim, E. | Lee, J H. | Park, S H. | Park, S J. | Rho, S H. | Crystal structure | Gkap | Pdz | Shank
