1qk7

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk7 OCA], [http://www.ebi.ac.uk/pdbsum/1qk7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qk7 RCSB]</span>
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[[Category: shl-i]]
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Revision as of 20:14, 30 March 2008


PDB ID 1qk7

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Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR


Overview

The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.

About this Structure

1QK7 is a Single protein structure of sequence from Ornithoctonus huwena. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR., Lu S, Liang S, Gu X, J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779

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