1qp5
From Proteopedia
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|PDB= 1qp5 |SIZE=350|CAPTION= <scene name='initialview01'>1qp5</scene>, resolution 2.6Å | |PDB= 1qp5 |SIZE=350|CAPTION= <scene name='initialview01'>1qp5</scene>, resolution 2.6Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qp5 OCA], [http://www.ebi.ac.uk/pdbsum/1qp5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qp5 RCSB]</span> | ||
}} | }} | ||
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[[Category: Timsit, Y.]] | [[Category: Timsit, Y.]] | ||
[[Category: Vilbois, E.]] | [[Category: Vilbois, E.]] | ||
| - | [[Category: MG]] | ||
[[Category: base pair shift]] | [[Category: base pair shift]] | ||
[[Category: dna self-fitting]] | [[Category: dna self-fitting]] | ||
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[[Category: microsatellite instability]] | [[Category: microsatellite instability]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:16:58 2008'' |
Revision as of 20:16, 30 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BASE-PAIRING SHIFT IN A DODECAMER CONTAINING A (CA)N TRACT
Overview
the crystal packing of the B-DNA dodecamer d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of double helices with specific base-backbone interactions in the major groove. Cooling the crystals below -10 degrees C stabilizes a new conformational state with a long-range sequence-dependent one-step shift in the major-groove base pairing. The tilt of the bases leads to the disruption of the Watson-Crick pairing in the major groove and to the formation of interactions with the 5' neighbour of their complement. This alteration propagates along the helical axis over more than half a turn. As a result, the molecular structure is normal when seen from the minor groove side and mismatched in the major groove. Comparison with a parent isomorphous dodecamer structure corresponding to the codon 10-13 of the c-Ha-ras proto-oncogene show that this new structural feature is sequence dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are involved both in recombination and in transcription, this new recognition pattern should be considered in the analysis of the various processes involving the reading of the genetic information.
About this Structure
1QP5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Base-pairing shift in the major groove of (CA)n tracts by B-DNA crystal structures., Timsit Y, Vilbois E, Moras D, Nature. 1991 Nov 14;354(6349):167-70. PMID:1944598
Page seeded by OCA on Sun Mar 30 23:16:58 2008
