This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3nz3

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 00:08, 5 August 2016

Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae

Structural highlights

3nz3 is a 1 chain structure with sequence from Strr6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	spr1345 (STRR6)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The surface protein Spr1345 from Streptococcus pneumoniae R6 is a 22-kDa mucin-binding protein (MucBP) involved in adherence and colonization of the human lung and respiratory tract. It is composed of a mucin-binding domain (MucBD) and a proline-rich domain (PRD) followed by an LPxTG motif, which is recognized and cleaved by sortase, resulting in a mature form of 171 residues (MF171) that is anchored to the cell wall. We found that the MucBD alone possesses comparable in vitro mucin-binding affinity to the mature form, and can be specifically enriched at the surface of human lung carcinoma A549 cells. Using single-wavelength anomalous dispersion (SAD) phasing method with the iodine signals, we solved the crystal structure of the MucBD at 2.0A resolution, the first structure of MucBDs from pathogenic bacteria. The overall structure adopts an immunoglobulin-like fold with an elongated rod-like shape, composed of six anti-parallel beta-strands and a long loop. Structural comparison suggested that the conserved C-terminal moiety may participate in the recognition of mucins. These findings provided structural insights into host-pathogen interaction mediated by mucins, which might be useful for designing novel vaccines and antibiotic drugs against human diseases caused by pneumococci.

Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae.,Du Y, He YX, Zhang ZY, Yang YH, Shi WW, Frolet C, Guilmi AM, Vernet T, Zhou CZ, Chen Y J Struct Biol. 2011 Apr;174(1):252-7. Epub 2010 Nov 3. PMID:21055474^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Du Y, He YX, Zhang ZY, Yang YH, Shi WW, Frolet C, Guilmi AM, Vernet T, Zhou CZ, Chen Y. Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae. J Struct Biol. 2011 Apr;174(1):252-7. Epub 2010 Nov 3. PMID:21055474 doi:10.1016/j.jsb.2010.10.016

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nz3"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae==
 <StructureSection load='3nz3' size='340' side='right' caption='[[3nz3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nz3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NZ3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nz3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NZ3 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spr1345 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spr1345 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nz3 RCSB], [http://www.ebi.ac.uk/pdbsum/3nz3 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nz3 OCA], [http://pdbe.org/3nz3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nz3 RCSB], [http://www.ebi.ac.uk/pdbsum/3nz3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nz3 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3nz3" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Streptococcus pneumoniae]]
+[[Category: Strr6]]
 [[Category: Chen, Y]]
 [[Category: Du, Y]]

3nz3

From Proteopedia

Revision as of 00:08, 5 August 2016

Crystal structure of the mucin-binding domain of Spr1345 from Streptococcus pneumoniae

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox