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1qye

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|PDB= 1qye |SIZE=350|CAPTION= <scene name='initialview01'>1qye</scene>, resolution 2.10&Aring;
|PDB= 1qye |SIZE=350|CAPTION= <scene name='initialview01'>1qye</scene>, resolution 2.10&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=CDY:2-CHLORODIDEOXYADENOSINE'>CDY</scene> and <scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>
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|LIGAND= <scene name='pdbligand=CDY:2-CHLORODIDEOXYADENOSINE'>CDY</scene>, <scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= TRA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 Canis lupus familiaris])
|GENE= TRA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 Canis lupus familiaris])
 +
|DOMAIN=
 +
|RELATEDENTRY=[[1qy5|1QY5]], [[1qy8|1QY8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qye OCA], [http://www.ebi.ac.uk/pdbsum/1qye PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qye RCSB]</span>
}}
}}
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[[Category: Nicchitta, C V.]]
[[Category: Nicchitta, C V.]]
[[Category: Soldano, K L.]]
[[Category: Soldano, K L.]]
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[[Category: CDY]]
 
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[[Category: M2M]]
 
[[Category: 2-chlorodideoxyadenosine]]
[[Category: 2-chlorodideoxyadenosine]]
[[Category: 2cldda]]
[[Category: 2cldda]]
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[[Category: hsp90]]
[[Category: hsp90]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:44:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:20:43 2008''

Revision as of 20:20, 30 March 2008


PDB ID 1qye

Drag the structure with the mouse to rotate
, resolution 2.10Å
Ligands: ,
Gene: TRA1 (Canis lupus familiaris)
Related: 1QY5, 1QY8


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with 2-chlorodideoxyadenosine


Overview

GRP94, the endoplasmic reticulum (ER) paralog of the chaperone Hsp90, plays an essential role in the structural maturation or secretion of a subset of proteins destined for transport to the cell surface, such as the Toll-like receptors 2 and 4, and IgG, respectively. GRP94 differs from cytoplasmic Hsp90 by exhibiting very weak ATP binding and hydrolysis activity. GRP94 also binds selectively to a series of substituted adenosine analogs. The high resolution crystal structures at 1.75-2.1 A of the N-terminal and adjacent charged domains of GRP94 in complex with N-ethylcarboxamidoadenosine, radicicol, and 2-chlorodideoxyadenosine reveals a structural mechanism for ligand discrimination among hsp90 family members. The structures also identify a putative subdomain that may act as a ligand-responsive switch. The residues of the charged region fold into a disordered loop whose termini are ordered and continue the twisted beta sheet that forms the structural core of the N-domain. This continuation of the beta sheet past the charged domain suggests a structural basis for the association of the N-terminal and middle domains of the full-length chaperone.

About this Structure

1QYE is a Single protein structure of sequence from Canis lupus familiaris. Full crystallographic information is available from OCA.

Reference

Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation., Soldano KL, Jivan A, Nicchitta CV, Gewirth DT, J Biol Chem. 2003 Nov 28;278(48):48330-8. Epub 2003 Sep 11. PMID:12970348

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