3qb5

From Proteopedia

(Difference between revisions)

Revision as of 15:52, 25 December 2014

Human C3PO complex in the presence of MnSO4

Structural highlights

3qb5 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Related:	3pja
Gene:	TSN (Homo sapiens), TSNAX, TRAX (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[TSN_HUMAN] DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.^[1] ^[2] Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.^[3] ^[4] [TSNAX_HUMAN] Acts in combination with TSN as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC). Possible role in spermatogenesis.^[5] ^[6]

Publication Abstract from PubMed

Assembly of the RNA-induced silencing complex (RISC) consists of loading duplex (guide-passenger) siRNA onto Argonaute (Ago2) and removing the passenger strand. Ago2 contributes critically to RISC activation by nicking the passenger strand. Here we reconstituted duplex siRNA-initiated RISC activity using recombinant human Ago2 (hAgo2) and C3PO, indicating that C3PO has a critical role in hAgo2-RISC activation. Consistently, genetic depletion of C3PO compromised RNA silencing in mammalian cells. We determined the crystal structure of hC3PO, which reveals an asymmetric octamer barrel consisting of six translin and two TRAX subunits. This asymmetric assembly is critical for the function of C3PO as an endonuclease that cleaves RNA at the interior surface. The current work supports a Dicer-independent mechanism for human RISC activation, in which Ago2 directly binds duplex siRNA and nicks the passenger strand, and then C3PO activates RISC by degrading the Ago2-nicked passenger strand.

Structure of C3PO and mechanism of human RISC activation.,Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H Nat Struct Mol Biol. 2011 May 8. PMID:21552258^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K. The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. J Biol Chem. 1997 Apr 25;272(17):11402-7. PMID:9111049
↑ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H. Structure of C3PO and mechanism of human RISC activation. Nat Struct Mol Biol. 2011 May 8. PMID:21552258 doi:10.1038/nsmb.2032
↑ Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K. The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. J Biol Chem. 1997 Apr 25;272(17):11402-7. PMID:9111049
↑ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H. Structure of C3PO and mechanism of human RISC activation. Nat Struct Mol Biol. 2011 May 8. PMID:21552258 doi:10.1038/nsmb.2032
↑ Bray JD, Chennathukuzhi VM, Hecht NB. Identification and characterization of cDNAs encoding four novel proteins that interact with translin associated factor-X. Genomics. 2002 Jun;79(6):799-808. PMID:12036294 doi:10.1006/geno.2002.6779
↑ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H. Structure of C3PO and mechanism of human RISC activation. Nat Struct Mol Biol. 2011 May 8. PMID:21552258 doi:10.1038/nsmb.2032
↑ Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H. Structure of C3PO and mechanism of human RISC activation. Nat Struct Mol Biol. 2011 May 8. PMID:21552258 doi:10.1038/nsmb.2032

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qb5"

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qb5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qb5 RCSB], [http://www.ebi.ac.uk/pdbsum/3qb5 PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/TSN_HUMAN TSN_HUMAN]] DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.<ref>PMID:9111049</ref> <ref>PMID:21552258</ref>   Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.<ref>PMID:9111049</ref> <ref>PMID:21552258</ref>  [[http://www.uniprot.org/uniprot/TSNAX_HUMAN TSNAX_HUMAN]] Acts in combination with TSN as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC). Possible role in spermatogenesis.<ref>PMID:12036294</ref> <ref>PMID:21552258</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

3qb5

From Proteopedia

Revision as of 15:52, 25 December 2014

Human C3PO complex in the presence of MnSO4

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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