1r3o
From Proteopedia
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|PDB= 1r3o |SIZE=350|CAPTION= <scene name='initialview01'>1r3o</scene>, resolution 1.90Å | |PDB= 1r3o |SIZE=350|CAPTION= <scene name='initialview01'>1r3o</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=LC:L-CYTIDINE-5'-MONOPHOSPHATE'>LC</scene>, <scene name='pdbligand=LG:L-GUANOSINE-5'-MONOPHOSPHATE'>LG</scene>, <scene name='pdbligand=LHU:L-URIDINE-5'-MONOPHOSPHATE'>LHU</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r3o OCA], [http://www.ebi.ac.uk/pdbsum/1r3o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r3o RCSB]</span> | ||
}} | }} | ||
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[[Category: l-rna]] | [[Category: l-rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:22:48 2008'' |
Revision as of 20:22, 30 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution
Overview
Nucleic acid molecules in the mirror image or L-configuration are unknown in nature and are extraordinarily resistant to biological degradation. The identification of functional L-oligonucleotides called Spiegelmers offers a novel approach for drug discovery based on RNA. The sequence r(CUGGGCGG).r(CCGCCUGG) was chosen as a model system for structural analysis of helices in the L-configuration as the structure of the D-form of this sequence has previously been determined in structural studies of 5S RNA domains, in particular domain E of the Thermus flavus 5S rRNA [Perbandt et al. (2001), Acta Cryst. D57, 219-224]. Unexpectedly, the results of crystallization trials showed little similarity between the D- and the L-forms of the duplex in either the crystallization hits or the diffraction performance. The crystal structure of this L-RNA duplex has been determined at 1.9 A resolution with R(work) and R(free) of 23.8 and 28.6%, respectively. The crystals belong to space group R32, with unit-cell parameters a = 45.7, c = 264.6 A. Although there are two molecules in the asymmetric unit rather than one, the structure of the L-form arranges helical pairs in a head-to-tail fashion to form pseudo-continuous infinite helices in the crystal as in the D-form. On the other hand, the wobble-like G.C(+) base pair seen in the D-RNA analogue does not appear in the L-RNA duplex, which forms a regular double-helical structure with typical Watson-Crick base pairing.
About this Structure
1R3O is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
First look at RNA in L-configuration., Vallazza M, Perbandt M, Klussmann S, Rypniewski W, Einspahr HM, Erdmann VA, Betzel Ch, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):1-7. Epub 2003 Dec, 18. PMID:14684885
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