1r74
From Proteopedia
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|PDB= 1r74 |SIZE=350|CAPTION= <scene name='initialview01'>1r74</scene>, resolution 2.55Å | |PDB= 1r74 |SIZE=350|CAPTION= <scene name='initialview01'>1r74</scene>, resolution 2.55Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | + | |LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Glycine_N-methyltransferase Glycine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.20 2.1.1.20] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycine_N-methyltransferase Glycine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.20 2.1.1.20] </span> |
|GENE= GNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= GNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r74 OCA], [http://www.ebi.ac.uk/pdbsum/1r74 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r74 RCSB]</span> | ||
}} | }} | ||
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[[Category: Pakhomova, S.]] | [[Category: Pakhomova, S.]] | ||
[[Category: Wagner, C.]] | [[Category: Wagner, C.]] | ||
| - | [[Category: BME]] | ||
| - | [[Category: CIT]] | ||
[[Category: glycine n-methyltransferase]] | [[Category: glycine n-methyltransferase]] | ||
[[Category: human]] | [[Category: human]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:24:14 2008'' |
Revision as of 20:24, 30 March 2008
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| , resolution 2.55Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | GNMT (Homo sapiens) | ||||||
| Activity: | Glycine N-methyltransferase, with EC number 2.1.1.20 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Human Glycine N-Methyltransferase
Contents |
Overview
Glycine N-methyltransferases (GNMTs) from three mammalian sources were compared with respect to their crystal structures and kinetic parameters. The crystal structure for the rat enzyme was published previously. Human and mouse GNMT were expressed in Escherichia coli in order to determine their crystal structures. Mouse GNMT was crystallized in two crystal forms, a monoclinic form and a tetragonal form. Comparison of the three structures reveals subtle differences, which may relate to the different kinetic properties of the enzymes.The flexible character of several loops surrounding the active site, along with an analysis of the active site boundaries, indicates that the observed conformations of human and mouse GNMTs are more open than that of the rat enzyme. There is an increase in kcat when going from rat to mouse to human, suggesting a correlation with the increased flexibility of some structural elements of the respective enzymes.
Disease
Known disease associated with this structure: Glycine N-methyltransferase deficiency OMIM:[606628]
About this Structure
1R74 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes., Pakhomova S, Luka Z, Grohmann S, Wagner C, Newcomer ME, Proteins. 2004 Nov 1;57(2):331-7. PMID:15340920
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