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1rb1

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|ACTIVITY=
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|RELATEDENTRY=[[1rb4|1RB4]], [[1rb5|1RB5]], [[1rb6|1RB6]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rb1 OCA], [http://www.ebi.ac.uk/pdbsum/1rb1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rb1 RCSB]</span>
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[[Category: peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:49:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:39 2008''

Revision as of 20:25, 30 March 2008

Image:1rb1.jpg


PDB ID 1rb1

Drag the structure with the mouse to rotate
, resolution 1.9Å
Related: 1RB4, 1RB5, 1RB6


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TRIGONAL AUTOMATIC SOLUTION


Overview

Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.

About this Structure

1RB1 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Automated protein crystal structure determination using ELVES., Holton J, Alber T, Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:14752198

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