1rgv
From Proteopedia
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|PDB= 1rgv |SIZE=350|CAPTION= <scene name='initialview01'>1rgv</scene>, resolution 2.90Å | |PDB= 1rgv |SIZE=350|CAPTION= <scene name='initialview01'>1rgv</scene>, resolution 2.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR CLUSTER'>SF4</scene> | + | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rgv OCA], [http://www.ebi.ac.uk/pdbsum/1rgv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rgv RCSB]</span> | ||
}} | }} | ||
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[[Category: Unciuleac, M.]] | [[Category: Unciuleac, M.]] | ||
[[Category: Warkentin, E.]] | [[Category: Warkentin, E.]] | ||
| - | [[Category: SF4]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:27:59 2008'' |
Revision as of 20:27, 30 March 2008
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| , resolution 2.90Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Ferredoxin from Thauera aromatica
Overview
4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a central enzyme in the metabolism of phenolic compounds in anaerobic bacteria. The enzyme catalyzes the reductive removal of the phenolic hydroxyl group from 4-hydroxybenzoyl-CoA, yielding benzoyl-CoA and water. 4-HBCR belongs to the xanthine oxidase (XO) family of molybdenum enzymes which occur as heterodimers, (alphabetagamma)(2). 4-HBCR contains two molybdopterins, four [2Fe-2S] and two [4Fe-4S] clusters and two FADs. A low-potential Allochromatium vinosum-type ferredoxin containing two [4Fe-4S] clusters serves as an in vivo electron donor for 4-HBCR. In this work, the oxygen-sensitive proteins 4-HBCR and the ferredoxin (TaFd) from Thauera aromatica were crystallized under anaerobic conditions. 4-HBCR crystallized with PEG 4000 and MPD as precipitant diffracted to about 1.6 A resolution and the crystals were highly suitable for X-ray structure analysis. Crystals of TaFd were obtained with (NH(4))(3)PO(4) as precipitant and revealed a solvent content of 77%, which is remarkably high for a small soluble protein. The structure of TaFd was solved at 2.9 A resolution by the molecular-replacement method using the highly related structure of the ferredoxin (CvFd) from A. vinosum as a model. Structural changes between the two ferredoxins around the [4Fe-4S] cluster can be correlated with their different redox potentials.
About this Structure
1RGV is a Single protein structure of sequence from Thauera aromatica. Full crystallographic information is available from OCA.
Reference
Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin., Unciuleac M, Boll M, Warkentin E, Ermler U, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):388-91. Epub 2004, Jan 23. PMID:14747735
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