This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1rzx
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1rzx |SIZE=350|CAPTION= <scene name='initialview01'>1rzx</scene>, resolution 2.1Å | |PDB= 1rzx |SIZE=350|CAPTION= <scene name='initialview01'>1rzx</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene> | + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= par-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | |GENE= par-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rzx OCA], [http://www.ebi.ac.uk/pdbsum/1rzx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rzx RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Prehoda, K E.]] | [[Category: Prehoda, K E.]] | ||
[[Category: Volkman, F B.]] | [[Category: Volkman, F B.]] | ||
| - | [[Category: ACE]] | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:25 2008'' |
Revision as of 20:35, 30 March 2008
| |||||||
| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | par-6 (Drosophila melanogaster) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a Par-6 PDZ-peptide Complex
Overview
Regulation of protein interaction domains is required for cellular signaling dynamics. Here, we show that the PDZ protein interaction domain from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42. Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately 13-fold. Par-6 PDZ regulation is required for function as mutational disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in polarized MDCK epithelial cells. Structural analysis reveals that the free PDZ domain has several deviations from the canonical PDZ conformation that account for its low ligand affinity. Regulation results from a Cdc42-induced conformational transition in the CRIB-PDZ module that causes the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled CRIB and PDZ architecture of Par-6 reveals how simple binding domains can be combined to yield complex regulation.
About this Structure
1RZX is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition., Peterson FC, Penkert RR, Volkman BF, Prehoda KE, Mol Cell. 2004 Mar 12;13(5):665-76. PMID:15023337
Page seeded by OCA on Sun Mar 30 23:35:25 2008
