1s5h
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1s5h |SIZE=350|CAPTION= <scene name='initialview01'>1s5h</scene>, resolution 2.20Å | |PDB= 1s5h |SIZE=350|CAPTION= <scene name='initialview01'>1s5h</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= KCSA, SKC1, SCO7660, SC10F4.33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Streptomyces coelicolor, and Streptomyces lividans]) | |GENE= KCSA, SKC1, SCO7660, SC10F4.33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Streptomyces coelicolor, and Streptomyces lividans]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5h OCA], [http://www.ebi.ac.uk/pdbsum/1s5h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s5h RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Mackinnon, R.]] | [[Category: Mackinnon, R.]] | ||
[[Category: Zhou, M.]] | [[Category: Zhou, M.]] | ||
| - | [[Category: DGA]] | ||
| - | [[Category: F09]] | ||
| - | [[Category: K]] | ||
[[Category: ion occupancy]] | [[Category: ion occupancy]] | ||
[[Category: k+ channel]] | [[Category: k+ channel]] | ||
| Line 33: | Line 33: | ||
[[Category: selectivity filter]] | [[Category: selectivity filter]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:37:39 2008'' |
Revision as of 20:37, 30 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | KCSA, SKC1, SCO7660, SC10F4.33 (Streptomyces coelicolor, and Streptomyces lividans) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Potassium Channel Kcsa-Fab Complex T75C mutant in K+
Overview
The selectivity filter of K(+) channels is comprised of a linear queue of four equal-spaced ion-binding sites spanning a distance of 12A. Each site is formed of eight oxygen atoms from the protein. The first three sites, numbered 1-3 from the extracellular side, are made of exclusively main-chain carbonyl oxygen atoms. The fourth site, closest to the intracellular side, is made of four main-chain carbonyl oxygen atoms and four threonine side-chain hydroxyl oxygen atoms. Here we characterize the effects of mutating the threonine to cysteine on the distribution of ions in the selectivity filter and on the conduction of ions through the filter. The mutation influences the occupancy of K(+) at sites 2 and 4 and it reduces the maximum rate of conduction in the limit of high K(+) concentration. The mutation does not affect the conduction of Rb(+). These results can be understood in the context of a conduction mechanism in which a pair of K ions switch between energetically balanced 1,3 and 2,4 configurations.
About this Structure
1S5H is a Single protein structure of sequence from Mus musculus and Streptomyces coelicolor, and streptomyces lividans. Full crystallographic information is available from OCA.
Reference
A mutant KcsA K(+) channel with altered conduction properties and selectivity filter ion distribution., Zhou M, MacKinnon R, J Mol Biol. 2004 May 7;338(4):839-46. PMID:15099749
Page seeded by OCA on Sun Mar 30 23:37:39 2008
