1sgv
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] </span> |
|GENE= TRUB, RV2793C, MT2862.1, MTV002.58C, MB2816C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]) | |GENE= TRUB, RV2793C, MT2862.1, MTV002.58C, MB2816C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]) | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02573 PseudoU_synth_EcTruB], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK03287 truB]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sgv OCA], [http://www.ebi.ac.uk/pdbsum/1sgv PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1sgv RCSB]</span> | ||
}} | }} | ||
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[[Category: trna modification]] | [[Category: trna modification]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:05:01 2008'' |
Revision as of 04:05, 26 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | TRUB, RV2793C, MT2862.1, MTV002.58C, MB2816C (Mycobacterium tuberculosis) | ||||||
| Activity: | Pseudouridylate synthase, with EC number 4.2.1.70 | ||||||
| Domains: | PseudoU_synth_EcTruB, truB | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)
Overview
The three-dimensional structure of the RNA-modifying enzyme, psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis, is reported. The 1.9-A resolution crystal structure reveals the enzyme, free of substrate, in two distinct conformations. The structure depicts an interesting mode of protein flexibility involving a hinged bending in the central beta-sheet of the catalytic module. Key parts of the active site cleft are also found to be disordered in the substrate-free form of the enzyme. The hinge bending appears to act as a clamp to position the substrate. Our structural data furthers the previously proposed mechanism of tRNA recognition. The present crystal structure emphasizes the significant role that protein dynamics must play in tRNA recognition, base flipping, and modification.
About this Structure
1SGV is a Single protein structure of sequence from Mycobacterium tuberculosis. This structure supersedes the now removed PDB entry 1S71. Full crystallographic information is available from OCA.
Reference
Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft., Chaudhuri BN, Chan S, Perry LJ, Yeates TO, J Biol Chem. 2004 Jun 4;279(23):24585-91. Epub 2004 Mar 17. PMID:15028724
Page seeded by OCA on Wed Mar 26 06:05:01 2008
Categories: Mycobacterium tuberculosis | Pseudouridylate synthase | Single protein | Chan, S. | Chaudhuri, B N. | Perry, L J. | TBSGC, TB Structural Genomics Consortium. | Yeates, T O. | Hinged motion | Protein structure initiative | Psi | Structural genomic | Tb structural genomics consortium | Tbsgc | Trna modification
