1sh4
From Proteopedia
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|PDB= 1sh4 |SIZE=350|CAPTION= <scene name='initialview01'>1sh4</scene> | |PDB= 1sh4 |SIZE=350|CAPTION= <scene name='initialview01'>1sh4</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1nx7|1nx7]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sh4 OCA], [http://www.ebi.ac.uk/pdbsum/1sh4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sh4 RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Wu, H.]] | [[Category: Wu, H.]] | ||
[[Category: Zhang, Q.]] | [[Category: Zhang, Q.]] | ||
| - | [[Category: HEM]] | ||
[[Category: five helix]] | [[Category: five helix]] | ||
[[Category: five sheet]] | [[Category: five sheet]] | ||
[[Category: heme ring]] | [[Category: heme ring]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:41:58 2008'' |
Revision as of 20:41, 30 March 2008
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| Ligands: | |||||||
| Related: | 1nx7
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H
Overview
A comparative study on the solution structures of bovine microsomal cytochrome b5 (Tb5) and the mutant V45H has been achieved by 1D and 2D 1H-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by approximately 3 degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.
About this Structure
1SH4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H., Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX, Protein Sci. 2004 Aug;13(8):2161-9. PMID:15273310
Page seeded by OCA on Sun Mar 30 23:41:58 2008
Categories: Bos taurus | Single protein | Wu, H. | Zhang, Q. | Five helix | Five sheet | Heme ring
