This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1sjm
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1sjm |SIZE=350|CAPTION= <scene name='initialview01'>1sjm</scene>, resolution 1.40Å | |PDB= 1sjm |SIZE=350|CAPTION= <scene name='initialview01'>1sjm</scene>, resolution 1.40Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span> |
|GENE= NIRK, NIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 Alcaligenes faecalis]) | |GENE= NIRK, NIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 Alcaligenes faecalis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1j9q|1J9Q]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjm OCA], [http://www.ebi.ac.uk/pdbsum/1sjm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sjm RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Rosell, F I.]] | [[Category: Rosell, F I.]] | ||
[[Category: Tocheva, E I.]] | [[Category: Tocheva, E I.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: NO2]] | ||
| - | [[Category: TRS]] | ||
[[Category: copper]] | [[Category: copper]] | ||
[[Category: nitrite]] | [[Category: nitrite]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:42:56 2008'' |
Revision as of 20:42, 30 March 2008
| |||||||
| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | NIRK, NIR (Alcaligenes faecalis) | ||||||
| Activity: | Nitrite reductase (NO-forming), with EC number 1.7.2.1 | ||||||
| Related: | 1J9Q
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nitrite bound copper containing nitrite reductase
Overview
A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions.
About this Structure
1SJM is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.
Reference
Side-on copper-nitrosyl coordination by nitrite reductase., Tocheva EI, Rosell FI, Mauk AG, Murphy ME, Science. 2004 May 7;304(5672):867-70. PMID:15131305
Page seeded by OCA on Sun Mar 30 23:42:56 2008
