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4j7y

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Revision as of 15:08, 5 August 2016

Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis

Structural highlights

4j7y is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	2uui, 2uuh, 4j7t
Gene:	LTC4S (HUMAN)
Activity:	Leukotriene-C(4) synthase, with EC number 4.4.1.20
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[LTC4S_HUMAN] Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency) [MIM:246530]. LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.

Function

[LTC4S_HUMAN] Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4.

Publication Abstract from PubMed

Leukotriene (LT) C4 synthase (LTC4S) catalyzes the conjugation of the fatty acid LTA4 with the tripeptide GSH to produce LTC4, the parent compound of the cysteinyl-leukotrienes, important mediators of asthma. Here we mutated Trp116 in human LTC4S, a residue proposed to play a key role in substrate binding, into an Ala or Phe. Biochemical and structural characterization of these mutants along with crystal structures of the wild type and mutated enzymes in complex with three product analogs, viz. S-hexyl-, 4- phenyl-butyl-, and 2-hydroxy-4-phenyl-butyl-glutathione, provide new insights to binding of substrates and product, identifies a new conformation of the GSH moiety at the active site, and suggests a route for product release, aided by Trp116.

Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism.,Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggstrom JZ J Biol Chem. 2013 Dec 23. PMID:24366866^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggstrom JZ. Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism. J Biol Chem. 2013 Dec 23. PMID:24366866 doi:http://dx.doi.org/10.1074/jbc.M113.534628

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4j7y"

@@ Line 1: / Line 1: @@
 ==Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis==
 <StructureSection load='4j7y' size='340' side='right' caption='[[4j7y]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LTC4S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Leukotriene-C(4)_synthase Leukotriene-C(4) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.20 4.4.1.20] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j7y RCSB], [http://www.ebi.ac.uk/pdbsum/4j7y PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j7y OCA], [http://pdbe.org/4j7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j7y RCSB], [http://www.ebi.ac.uk/pdbsum/4j7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j7y ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 21: / Line 22: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4j7y" style="background-color:#fffaf0;"></div>
 ==See Also==

4j7y

From Proteopedia

Revision as of 15:08, 5 August 2016

Human LTC4 synthase in complex with product analogs - implications for enzyme catalysis

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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