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1t44

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|PDB= 1t44 |SIZE=350|CAPTION= <scene name='initialview01'>1t44</scene>, resolution 2.00&Aring;
|PDB= 1t44 |SIZE=350|CAPTION= <scene name='initialview01'>1t44</scene>, resolution 2.00&Aring;
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>
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[[Category: structural protein]]
[[Category: structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:41:25 2008''

Revision as of 11:41, 23 March 2008

Image:1t44.gif


PDB ID 1t44

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Structural basis of actin sequestration by thymosin-B4: Implications for arp2/3 activation


Contents

Overview

The WH2 (Wiscott-Aldridge syndrome protein homology domain 2) repeat is an actin interacting motif found in monomer sequestering and filament assembly proteins. We have stabilized the prototypical WH2 family member, thymosin-beta4 (Tbeta4), with respect to actin, by creating a hybrid between gelsolin domain 1 and the C-terminal half of Tbeta4 (G1-Tbeta4). This hybrid protein sequesters actin monomers, severs actin filaments and acts as a leaky barbed end cap. Here, we present the structure of the G1-Tbeta4:actin complex at 2 A resolution. The structure reveals that Tbeta4 sequesters by capping both ends of the actin monomer, and that exchange of actin between Tbeta4 and profilin is mediated by a minor overlap in binding sites. The structure implies that multiple WH2 motif-containing proteins will associate longitudinally with actin filaments. Finally, we discuss the role of the WH2 motif in arp2/3 activation.

Disease

Known diseases associated with this structure: Myopathy, actin, congenital, with cores OMIM:[102610], Myopathy, actin, congenital, with excess of thin myofilaments OMIM:[102610], Myopathy, congenital, with fiber-type disporportion 1 OMIM:[102610], Myopathy, nemaline, 3 OMIM:[102610]

About this Structure

1T44 is a Protein complex structure of sequences from Homo sapiens, mus musculus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

Structural basis of actin sequestration by thymosin-beta4: implications for WH2 proteins., Irobi E, Aguda AH, Larsson M, Guerin C, Yin HL, Burtnick LD, Blanchoin L, Robinson RC, EMBO J. 2004 Sep 15;23(18):3599-608. Epub 2004 Aug 26. PMID:15329672

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