1a6j

From Proteopedia

Revision as of 13:40, 5 November 2007

NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN

Overview

The bacterial rpoN operon codes for sigma 54, which is the key sigma, factor that, under nitrogen starvation conditions, activates the, transcription of genes needed to assimilate ammonia and glutamate. The, rpoN operon contains several other open reading frames that are, cotranscribed with sigma 54. The product of one of these, the 17.9 kDa, protein IIANtr, is homologous to IIA proteins of the, phosphoenolpyruvate:sugar phosphotransferase (PTS) system. IIANtr, influences the transcription of sigma 54-dependent genes through an, unknown mechanism and may thereby provide a regulatory link between carbon, and nitrogen metabolism. Here we describe the 2.35 A X-ray structure of, Escherichia coli IIANtr. It is the first structure of a IIA enzyme from, the fructose-mannitol family of the PTS. The enzyme displays a novel fold, characterized by a central mixed parallel/anti-parallel beta-sheet, surrounded by six alpha-helices. The active site His73 is situated in a, shallow depression on the protein surface.

About this Structure

1A6J is a Single protein structure of sequence from Escherichia coli with SO4 and BME as ligands. Active as Protein-N(pi)-phosphohistidine--sugar phosphotransferase, with EC number 2.7.1.69 Structure known Active Site: . Full crystallographic information is available from OCA.

Reference

The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli., Bordo D, van Monfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Dijkstra BW, J Mol Biol. 1998 May 29;279(1):245-55. PMID:9636714

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