1t7x
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= AZGP1, ZAG, ZNGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= AZGP1, ZAG, ZNGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zag|1ZAG]], [[1t7v|1T7V]], [[1t7w|1T7W]], [[1t7y|1T7Y]], [[1t7z|1T7Z]], [[1t80|1T80]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7x OCA], [http://www.ebi.ac.uk/pdbsum/1t7x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t7x RCSB]</span> | ||
}} | }} | ||
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[[Category: Kennedy, M W.]] | [[Category: Kennedy, M W.]] | ||
[[Category: McDermott, L.]] | [[Category: McDermott, L.]] | ||
| - | [[Category: NAG]] | ||
[[Category: mhc class i homolog]] | [[Category: mhc class i homolog]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:34 2008'' |
Revision as of 20:52, 30 March 2008
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| , resolution 3.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | AZGP1, ZAG, ZNGP1 (Homo sapiens) | ||||||
| Related: | 1ZAG, 1T7V, 1T7W, 1T7Y, 1T7Z, 1T80
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Zn-alpha-2-glycoprotein; refolded CHO-ZAG PEG 400
Overview
Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. The previously reported 2.8 A crystal structure of ZAG isolated from human serum demonstrated the structural similarity between ZAG and class I major histocompatibility complex (MHC) molecules and revealed a non-peptidic ligand in the ZAG counterpart of the MHC peptide-binding groove. Here we present crystallographic studies to explore further the nature of the non-peptidic ligand in the ZAG groove. Comparison of the structures of several forms of recombinant ZAG, including a 1.95 A structure derived from ZAG expressed in insect cells, suggests that the non-peptidic ligand in the current structures and in the structure of serum ZAG is a polyethylene glycol (PEG), which is present in the crystallization conditions used. Further support for PEG binding in the ZAG groove is provided by the finding that PEG displaces a fluorophore-tagged fatty acid from the ZAG binding site. From these results we hypothesize that our purified forms of ZAG do not contain a bound endogenous ligand, but that the ZAG groove is capable of binding hydrophobic molecules, which may relate to its function.
About this Structure
1T7X is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein., Delker SL, West AP Jr, McDermott L, Kennedy MW, Bjorkman PJ, J Struct Biol. 2004 Nov;148(2):205-13. PMID:15477100
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