1t94
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= DINB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= DINB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t94 OCA], [http://www.ebi.ac.uk/pdbsum/1t94 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t94 RCSB]</span> | ||
}} | }} | ||
| Line 28: | Line 31: | ||
[[Category: Prakash, S.]] | [[Category: Prakash, S.]] | ||
[[Category: Uljon, S N.]] | [[Category: Uljon, S N.]] | ||
| - | [[Category: | + | [[Category: dna repair]] |
| + | [[Category: lesion bypass]] | ||
| + | [[Category: replication]] | ||
| + | [[Category: translesion dna synthesis]] | ||
| + | [[Category: y-family dna polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:53:04 2008'' |
Revision as of 20:53, 30 March 2008
| |||||||
| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | DINB (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the catalytic core of human DNA polymerase kappa
Overview
We present the crystal structure of the catalytic core of human DNA polymerase kappa (hPolkappa), the first structure of a human Y-family polymerase. hPolkappa is implicated in the proficient extension of mispaired primer termini on undamaged DNAs, and in the extension step of lesion bypass. The structure reveals a stubby "fingers" subdomain, which despite its small size appears to be tightly restrained with respect to a putative templating base. The structure also reveals a novel "thumb" subdomain that provides a basis for the importance of the N-terminal extension unique to hPolkappa. And, most surprisingly, the structure reveals the polymerase-associated domain (PAD) juxtaposed on the dorsal side of the "palm" subdomain, as opposed to the fingers subdomain. Together, these properties suggest that the hPolkappa active site is constrained at the site of the templating base and incoming nucleotide, but the polymerase is less constrained following translocation of the lesion.
About this Structure
1T94 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the catalytic core of human DNA polymerase kappa., Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK, Structure. 2004 Aug;12(8):1395-404. PMID:15296733
Page seeded by OCA on Sun Mar 30 23:53:04 2008
